We narrowed to 5,680 results for: SUP

1. lentiCRISPR v2-sgBAP1-1

   Plasmid

   #125837

   Purpose

   KO BAP1 gene

   Depositor

   Boyi Gan

   Article

   Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

   Insert

   BAP1 (BRCA1 associated protein 1) (BAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. LENTICRISPR-ALDOA_sgRNA1

   Plasmid

   #201590

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   ALDOA (ALDOA Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-GAL4-IL10-IRES-mCherry-PGK-BFP

   Plasmid

   #223209

   Purpose

   Lentiviral vector - mCherry reporter and IL10 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

   Depositor

   Wendell Lim

   Article

   Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

   Insert

   IL10 (IL10 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPR v2-sgBAP1-2

   Plasmid

   #125838

   Purpose

   KO BAP1 gene

   Depositor

   Boyi Gan

   Article

   Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

   Insert

   BAP1 (BRCA1 associated protein 1) (BAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_NESPylRS(WT)_hU6tRNAPyl

   Plasmid

   #223509

   Purpose

   PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

   Depositor

   Edward Lemke

   Article

   Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

   Inserts

   NES Pyrrolysyl-tRNA Synthetase (pylS Methanosarcina mazei)
   tRNA Pyl
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6 and U6

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB-Halo,IRES-eGFP

   Plasmid

   #164519

   Purpose

   All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)

   Depositor

   Mitchell Guttman

   Article

   Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVNP2.0-SpCas9

   Plasmid

   #206880

   Purpose

   Expresses FLAG-tagged SpCas9 fused to the N-terminus of Gag/GagPol harbouring an intervening phospholipase C-δ1 pleckstrin homology (PH) domain

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

   Insert

   SpCas9 (cas9 )
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTagGFP2-N SIN1

   Plasmid

   #124919

   Purpose

   For expression of wild-type SIN1-GFP

   Depositor

   Jin Chen

   Article

   Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

   Insert

   MAPKAP1 (MAPKAP1 Human)
   

   Use

   Tags

   TagGFP2

   Expression

   Mammalian

   Mutation

   Insert ORF was sirently mutated to be resistent t…

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. CD154-His-bio

   Plasmid

   #52337

   Purpose

   Expresses full-length Tumor necrosis factor ligand superfamily member 5 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   CD154 (CD40LG Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. shp63alpha pLKO.1 puro

    Plasmid

    #19120

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    small hairpin RNA against tumor protein p63 (TP63 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV p2GM AMPK alpha2hp1 alpha1hp1

    Plasmid

    #89492

    Purpose

    shRNA against AMPK alpha2 and alpha1 in mouse/human to knock down protein expression

    Depositor

    Russell Jones

    Article

    Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

    Insert

    2 hairpins against mouse/human AMPKalpha1 and alpha2
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (The GFP-Puro is from a PGK promoter; the shRN…

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

12. PB-Neo-TetO-Cdk12_Nterm_FlagHa

    Plasmid

    #127179

    Purpose

    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    

    Use

    Piggybac transposase vector

    Tags

    Flag- HA- Tandem Epitopes

    Expression

    Mammalian

    Mutation

    Promoter

    TetO Promoter

    Available since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. IL2R/hE-cadherin-cytotail

    Plasmid

    #45773

    Depositor

    Barry Gumbiner

    Article

    Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

    Insert

    IL2R/hE-cadherin-cytotail (IL2RA, CDH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Interleukin-2 receptor α subunit extracellular an…

    Promoter

    CMV

    Available since

    June 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

    Plasmid

    #184397

    Purpose

    Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Firefly luciferase beta-globin-PTC39
    

    Use

    CRISPR; Donor plasmid for hdr

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    PTC in beta-globin at amino acid position 39

    Promoter

    Tet-On 3G

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-H2BmGreenLantern

    Plasmid

    #177332

    Purpose

    To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

    Depositor

    Pantelis Tsoulfas, Gregory Petsko, Murray Blackmore

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    H2B (Hist1h2bq Rat)
    

    Use

    AAV

    Tags

    mGreenLantern

    Expression

    Mammalian

    Mutation

    This fusion protein was optimized to the Human co…

    Promoter

    CAG

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX MLKL(wt)--2A-mCherry-puro

    Plasmid

    #231974

    Purpose

    Tet inducible expression of DmrB-Mlkl with mCherry to induce WT Mlkl expression

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    MLKL WT construct with bi-cistronic mCherry (Mlkl Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1

    Plasmid

    #184398

    Purpose

    Donor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Renilla luciferase beta-globin-PTC39
    

    Use

    CRISPR; Donor plasmid for hdr

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    PTC in beta-globin at amino acid position 39

    Promoter

    Tet-On 3G

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-puro/Flag PD-L1 N219Q

    Plasmid

    #121452

    Purpose

    expression of huamn PD-L1 mutant in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N219Q

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV_iRFP_P2AT2A_mScarlet-I_Myl9

    Plasmid

    #172474

    Purpose

    Mammalian expression of myosin light chain (Myl9) tagged with mScarlet-I and cytoplasmic iRFP as a reference marker.

    Depositor

    Sean R Collins

    Article

    Hadjitheodorou et al Nat Commun. 2021 Nov 16;12(1):6619. doi:

    Inserts

    iRFP670
    mScarlet-I-Myl9 (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha (with UCOE) and none (same transcript as…

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-puro/Flag PD-L1 N200Q

    Plasmid

    #121451

    Purpose

    expression of human PD-L1 mutant in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N200Q

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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