We narrowed to 13,635 results for: cdna

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pcDNA3-FLAG-MTOR-S2215Y

Plasmid

#69013

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

S2215Y

Promoter

CMV

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloAKAR2.2-T2A-EGFP

Plasmid

#216490

Purpose

Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.2

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-HiBiT-N

Plasmid

#215815

Purpose

Expresses N protein with HiBiT tag in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 HiBiT Nucleocapsid (N )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_535-1102 USP7

Plasmid

#131246

Purpose

Mammalian expression of the five USP7 UBL domains with an N-terminal Myc tag.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Expresses USP7 amino acids 535-1102. This truncat…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_1-560 USP7

Plasmid

#131244

Purpose

Mammalian expression of N-terminally Myc-tagged USP7, minus the UBL domains

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Expresses USP7 amino acids 1 - 560. This truncati…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-N-mEGFP-PARP15

Plasmid

#178000

Purpose

Expresses N-terminally mEGFP-tagged PARP15

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

PARP15 (PARP15 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 VDAC1-Linker-GFP

Plasmid

#211734

Purpose

Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the C-terminus of VDAC1 with a flexible linker.

Depositor

Roland Malli

Article

Pilic et al PLoS One. 2024 Oct 18;19(10):e0311107. d

Insert

Voltage-dependent anion channel 1 (VDAC1 )

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-PHLPP1 full length

Plasmid

#37100

Purpose

Expresses HA tagged, full-length human PHLPP1 in mammalian cells

Depositor

Alexandra Newton

Article

Warfel et al J Biol Chem. 2011 Jun 3;286(22):19777-88

Insert

PHLPP1 (PHLPP1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP

Plasmid

#194683

Purpose

CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_05-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/hChR2(H134R)-mCherry

Plasmid

#20938

Purpose

Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

CMV

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA-RanWT-mRFP1-polyA

Plasmid

#59750

Purpose

Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran-mRFP1

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

Plasmid

#194681

Purpose

CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_01-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJA2

Plasmid

#19492

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJA2 (DNAJA2 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA4c/His-Xpress-HA-hBIG2(WT)

Plasmid

#79432

Purpose

Expresses N-terminally His6, Xpress and HA-tagged BIG2(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

Insert

BIG2 (ARFGEF2 Human)

Tags

HA, His6, and Xpress

Expression

Mammalian

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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L4276 hproTGFb1 in pcDNA backbone

Plasmid

#244183

Purpose

Constitutive expression of human pro-TGF-beta1 (with its signal sequence)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

pro-TGF-beta1 (TGFB1 Human, Synthetic)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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CeNL(Ca2+)_110μ-pcDNA3

Plasmid

#111929

Purpose

Cyan color bright luminescent calcium indicator with low affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_110μ

Expression

Mammalian

Mutation

E31D, F92W, E104D. D133E at CaM

Promoter

CMV

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-neo (1795)

Plasmid

#41000

Depositor

Jonathon Pines

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615)

Plasmid

#115788

Purpose

Expresses human LRP5 with N-terminal 3xflag tag in mammalian cells, contains artificial signal sequence upstream of tag.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

LRP5 (LRP5 Human)

Tags

3xflag

Expression

Mammalian

Mutation

contains silent mutation that creates an internal…

Promoter

CMV

Available Since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Connexin43-GFP-APEX2

Plasmid

#49385

Purpose

Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

Connexin43 - EGFP - APEX2

Expression

Mammalian

Mutation

K14D, W41F, E112K, A134P on soybean APX; improved…

Promoter

CMV

Available Since

Nov. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-ExRai-AMPKAR

Plasmid

#192449

Purpose

ExRai-AMPKAR targeted to the lysosomal membrane.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

Lyso-ExRai-AMPKAR

Tags

Full-length LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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