We narrowed to 5,867 results for: nls

1. 3xnls-G-Ca-FLITS

   Plasmid

   #191463

   Purpose

   Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   G-Ca-FLITS
   

   Use

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-NLS-ZapCV2

   Plasmid

   #203664

   Purpose

   Expresses zinc sensor ZapCV2 in the nucleus of mammalian cells

   Depositor

   Amy Palmer

   Article

   Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

   Insert

   ZapCV2 Zinc sensor
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRIPZ (M)-HT-NLS

   Plasmid

   #82518

   Purpose

   Lentivirus vector. Expresses Halotag proteins with nuclear localization signal in mammalian cells.

   Depositor

   Xiaojun Ren

   Insert

   Nuclear localizaition sequence
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV_Efs_hSpCas9_NLS_FLAG-SV40

   Plasmid

   #97307

   Purpose

   AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   humanized S. pyogenes Cas9
   

   Use

   AAV and Mouse Targeting

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_EF1a-->GssmycLaG16LaG2_P2A_NLSIFP

   Plasmid

   #162228

   Purpose

   Lentiviral vector for the expression of Myc GFP nanbody LaG16LaG2 with IFP NLS in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2020 Oct 16;370(6514):327-331.

   Insert

   LaG16LaG2 and IFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti_Efs_hSpCas9_NLS_FLAG-WPRE

   Plasmid

   #97313

   Purpose

   Lentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Lentiviral and Mouse Targeting

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3E-nlsGFP no-pA

   Plasmid

   #80815

   Purpose

   nlsGFP without pA

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   p3E-CMV-nlsGFP no pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-P4:gs:nls:VPR

   Plasmid

   #135983

   Purpose

   Expresses the P4 coiled coil fused to the VPR transcriptional activation domain in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   P4 coiled-coil fused to the VPR activation domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. 2xNLS-pMJ915v2

   Plasmid

   #88916

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. AsCpf1-2NLS

    Plasmid

    #102565

    Purpose

    bacterial expression of AsCpf1-2NLS

    Depositor

    Jennifer Doudna

    Article

    Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

    Insert

    AsCpf1
    

    Use

    Tags

    6xHis-MBP-TEV and HA-2xSV40NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. BB1_23_dCAS9(3xFLAG_SV40NLS)

    Plasmid

    #161478

    Purpose

    BB1 encoding a FLAG tagged dCAS9 with a SV40 NLS

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Insert

    dCAS9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    FLAG Tag, SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJIT163-2NLSCas9

    Plasmid

    #53064

    Purpose

    Expression of rice codon-optimized Cas9 in plant cells

    Depositor

    Caixia Gao

    Article

    Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

    Insert

    Cas9
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    Rice codon optimized

    Promoter

    2x35S

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR

    Plasmid

    #81251

    Purpose

    Lentiviral construct for building stable cell lines expressing Cas9 via puromycin selection

    Depositor

    Timothy Lu

    Article

    Perli et al Science. 2016 Aug 18. pii: aag0511.

    Insert

    Cas9-3xNLS
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMVMagFRET-1-NLS

    Plasmid

    #50748

    Purpose

    Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

    Insert

    gene encoding for MagFRET-1 with NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAi14-GFPNLS-MC

    Plasmid

    #87114

    Purpose

    minicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    GFPNLSPpA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    nEF

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR_TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP

    Plasmid

    #162233

    Purpose

    Lentiviral vector for the expression of GFP nanobody LaG16LaG2 -IFP : TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    anti-GFP nanobody lag16lag2 and IFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR'CMVGFP_NLS_hRIF1(1-1308)

    Plasmid

    #23134

    Depositor

    Lifeng Xu

    Article

    Xu et al J Cell Biol. 2004 Dec 6. 167(5):819-30.

    Insert

    RIF1 deletion construct (RIF1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-21a_2xNLS_LbCpf1_protein_expression

    Plasmid

    #114366

    Purpose

    Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

    Insert

    2xNLS_LbCpf1
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

    Plasmid

    #79372

    Purpose

    Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    GCN4-sfGFP-p65-hsf1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. M_NLS 41kDa

    Plasmid

    #201341

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    (NLS)SV40A5-EGFP-2PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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