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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,374 results for: ccdB

Showing: 801 - 840 of 1374 results

  1. pOCC154

    Plasmid
    #118902
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    Use
    Tags
    TwinStrep, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118902-plasmid-map-sequence-id-232325

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  3. pOCC153

    Plasmid
    #118901
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    TwinStrep, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118901-plasmid-map-sequence-id-232312

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  4. pDrop

    Plasmid
    #184872
    Purpose
    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.
    Depositor
    Patrick Sobetzko
    Article
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    lacZalpha,ccdB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Jan. 3, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    184872-plasmid-map-sequence-id-364202

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  5. pAL3

    Plasmid
    #114673
    Purpose
    T7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.
    Depositor
    William Studier
    Article
    Studier et al Curr Protoc Mol Biol. 2018 Oct;124(1):e6
    Type
    Empty backbone
    Use
    Tags
    optional Ser-Gly-6His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    114673-plasmid-map-sequence-id-254326

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  20. pOCC111

    Plasmid
    #118906
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal sortase site (LPETGg) and HIS6
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-LPETGg-HIS6-stop-HindIII cassette
    Use
    Tags
    LPETGg, HIS6
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118906-plasmid-map-sequence-id-232320

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  21. mito NAD+ biosensor

    Plasmid
    #186791
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondria
    Depositor
    Michael Cohen
    Article
    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d
    Insert
    mito NAD+ biosensor
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186791-plasmid-map-sequence-id-368060

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  22. cytoplasmic NAD+ biosensor

    Plasmid
    #186787
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm
    Depositor
    Michael Cohen
    Article
    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d
    Insert
    cytoplasmic NAD+ biosensor
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186787-plasmid-map-sequence-id-368056

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  23. nuclear NAD+ biosensor

    Plasmid
    #186789
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus
    Depositor
    Michael Cohen
    Article
    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d
    Insert
    nuclear NAD+ biosensor
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186789-plasmid-map-sequence-id-368062

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  25. pOCC65

    Plasmid
    #118865
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease, mGFP
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118865-plasmid-map-sequence-id-232317

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  28. pOCC102

    Plasmid
    #118883
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C protease
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6-MBP, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118883-plasmid-map-sequence-id-232315

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  29. pOCC151

    Plasmid
    #118899
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal GST, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-GST-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    GST tag, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118899-plasmid-map-sequence-id-232310

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  31. pOCC5

    Plasmid
    #118863
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118863-plasmid-map-sequence-id-232276

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  38. pOCC7

    Plasmid
    #118876
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118876-plasmid-map-sequence-id-232237

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Showing: 801 - 840 of 1374 results