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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,239 results for: membrane gfp

Showing: 801 - 840 of 1239 results

  2. hSyn-HA-PGRN-LAMP1TM-IRES-GFP

    Plasmid
    #234877
    Purpose
    Lentiviral plasmid expressing HA-tagged human progranulin that is fused to the transmembrane domain and cytosolic tail of LAMP-1. Enables lysosomal delivery of progranulin without secretion.
    Depositor
    Andrew Arrant
    Article
    Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi
    Insert
    Progranulin (GRN Human)
    Use
    Lentiviral
    Tags
    HA tag and LAMP-1 transmembrane domain and cytoso…
    Expression
    Mammalian
    Mutation
    Promoter
    hSYn
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234877-plasmid-map-sequence-id-468254

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  9. pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)

    Plasmid
    #224497
    Purpose
    Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    Actin-Vhh-sfGFP-P2A-HA-tdiRFP-caax
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    224497-plasmid-map-sequence-id-447355

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  10. pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)

    Plasmid
    #224496
    Purpose
    Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    Actin-Vhh-sfGFP-P2A-HA-iRFP-caax
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    224496-plasmid-map-sequence-id-447065

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  11. pEGFP-N1-Letm1

    Plasmid
    #191937
    Purpose
    Expression of a Letm1 (Leucine Zipper And EF-Hand Containing Transmembrane Protein 1)- GFP chimera.
    Depositor
    Luca Scorrano
    Insert
    Letm1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    191937-plasmid-map-sequence-id-379512

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  15. trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

    Plasmid
    #184774
    Purpose
    Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.
    Depositor
    Zhiping Xie
    Article
    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10
    Insert
    proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Budding Yeast, Mouse)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    184774-plasmid-map-sequence-id-382498

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  20. CyTERM-mGFP

    Plasmid
    #62237
    Purpose
    ER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.
    Depositor
    Erik Snapp
    Article
    Costantini et al Traffic. 2012 May;13(5):643-9. doi: 10.1
    Insert
    CyTERM-mGFP
    Use
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    signal anchor of rabbit cytochrome p450 and monom…
    Promoter
    CMV
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    62237-plasmid-map-sequence-id-320621

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  21. GPD-roGFP

    Plasmid
    #49436
    Purpose
    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells
    Depositor
    Paul Schumacker
    Article
    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi
    Insert
    GPD-roGFP2
    Use
    Adenoviral
    Tags
    Glycerol phosphate dehydrogenase, 5' to roGF…
    Expression
    Mammalian
    Mutation
    C48S, Q80R, S147C, and Q204C in Clontech's G…
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49436-plasmid-map-sequence-id-251833

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  22. NET29b.Mm pEGFP-N2 (1327)

    Plasmid
    #62046
    Purpose
    mammalian expression of nuclear envelope transmembrane protein
    Depositor
    Eric Schirmer
    Insert
    NET29b (Tmem120b Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    62046-plasmid-map-sequence-id-101295

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  23. NET29A.Mm pEGFP-N2 (1326)

    Plasmid
    #62045
    Purpose
    mammalian expression of nuclear envelope transmembrane protein
    Depositor
    Eric Schirmer
    Insert
    NET29A (Tmem120a Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    62045-plasmid-map-sequence-id-101294

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  24. NET43 pEGFP-N2 (609)

    Plasmid
    #61991
    Purpose
    mammalian expression of nuclear envelope transmembrane protein
    Depositor
    Eric Schirmer
    Insert
    NET43 (NAT10 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only

  26. InsRA-eGFP

    Plasmid
    #79795
    Purpose
    Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.
    Depositor
    James Johnson
    Article
    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:
    Insert
    InsRA (INSR Human)
    Use
    Tags
    eGFP extracellular
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    79795-plasmid-map-sequence-id-149406

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  27. pUASt- Crb intraGFP

    Plasmid
    #67525
    Purpose
    Expression of transmembrane and the intracellular domains of Crb with GFP.
    Depositor
    Elisabeth Knust, Marta Llimargas Casanova
    Insert
    crumbs (crb Fly)
    Use
    Drosophila
    Tags
    GFP
    Expression
    Insect
    Mutation
    aa97-2103 deleted (extracellular domain)
    Promoter
    hsp70
    Available since
    Aug. 5, 2015
    Availability
    Academic Institutions and Nonprofits only

  36. pLTR.gp140/EGFP.Revdelta38/DsRed

    Plasmid
    #115775
    Purpose
    HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.
    Depositor
    Damian Purcell
    Article
    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37
    Insert
    Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
    Use
    Tags
    EGFP; dsReD
    Expression
    Mammalian
    Mutation
    gp140 uncleaved (1190-91aa, KR > TG; truncated…
    Promoter
    HIV-1 (LTR)
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115775-plasmid-map-sequence-id-224438

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Showing: 801 - 840 of 1239 results