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pBGO

Plasmid

#62671

Purpose

Donor vector for mFTH1 knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

FTH1

Use

Cre/Lox

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pAG191 MDFIC 3'UTR

Plasmid

#12018

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N22 3'UTR (MDFIC Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pAG147 BC072452 3'UTR

Plasmid

#12005

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N12 3'UTR (FAM107B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pAG228 TNKS2 3'UTR mut

Plasmid

#12031

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

TNKS2 3'UTR mut (TNKS2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pAG174 TNS3 3'UTR mut

Plasmid

#12032

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N7 3'UTR mut (TNS3 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pAG219 RIPK4 3'UTR mut

Plasmid

#12049

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N20 3'UTR mut (RIPK4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pAG72 PFTK1 3'UTR

Plasmid

#12028

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

PFTK1 3'UTR (CDK14 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pAG81 ZAP3 3'UTR mut

Plasmid

#12057

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N3 3'UTR mut (YLPM1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pAG87 DUXAP6 3'UTR mut

Plasmid

#12060

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N6 3'UTR mut (DUXAP6 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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UNK_ZnF1-3_pGEX

Plasmid

#219664

Purpose

Recombinant expression of UNK ZnF1-3 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al Nat Commun. 2024 Sep 27;15(1):8400. doi:

Insert

Unkempt Zinc Fingers 1-3

Use

Tags

GST and SBP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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UNK_ZnF4-6_pGEX

Plasmid

#219665

Purpose

Recombinant expression of UNK ZnF4-6 with dual-affinity tag

Depositor

Daniel Dominguez

Article

Harris et al Nat Commun. 2024 Sep 27;15(1):8400. doi:

Insert

Unkempt Zinc Fingers 4-6

Use

Tags

GST and SBP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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iNicSnFR12

Plasmid

#227142

Purpose

Genetically encoded fluorescent biosensor for nicotine detection

Depositor

Henry Lester

Article

Haloi et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Insert

iNicSnFR12

Use

Tags

6xHis-HA and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL758

Plasmid

#219504

Purpose

Express Eco3RT from a CAG promoter; also express an msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

Insert

msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences

Use

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pSLS.407

Plasmid

#187204

Purpose

Expresses retron Eco1 ncRNA v32, and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v32
Cas1 + Cas2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7/Lac

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSLS.408

Plasmid

#187205

Purpose

Expresses retron Eco1 ncRNA v35, and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35
Cas1 + Cas2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7/Lac

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4D41

Plasmid

#185841

Purpose

Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10CF72 (B11)

Plasmid

#185859

Purpose

Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10CF72 (B9)

Plasmid

#185858

Purpose

Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10CF72

Plasmid

#185857

Purpose

Testing the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10E1B

Plasmid

#185856

Purpose

Testing the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge