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Plasmid#225064PurposeExpression of HalM-modified HalA2 in human cellsDepositorInsertHalA2
UseTagsMultiple tags due to polycistronic expressionExpressionMammalianMutationWTPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG
Plasmid#217321PurposePurification of autocleavage-deficient Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationS969APromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac dual_Human full length CDK7(W132R, S164D, T170E)
Plasmid#217013PurposeExpression of CDK7 in insect cellsDepositorInsertCDK7(W132R, S164D, T170E) (CDK7 Human)
UseTagsTEV cleavable his tagExpressionInsectMutationCDK7(W132R, S164D, T170E)PromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
Plasmid#79030PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
UseTagsOneSTrEP-FLAGExpressionInsectMutationdeleted amino acid 294-515 from frican green monk…PromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)
Plasmid#48294DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual His6 MBP N10 TEV LIC cloning vector (5C)
Plasmid#30123DepositorTypeEmpty backboneUseTagsHis6-MBP-N10-TEVExpressionInsectMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST (W18A/L21A; deltaLIR)
Plasmid#223766PurposeExpression of recombinant protein for purificationDepositorInsertBNIP3 soluble part (BNIP3 Human)
UseTagsEGFP-TEV-GSTExpressionInsectMutationW18A/L21A; deletion of LIRPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T
Plasmid#225063PurposeExpression of CylM-modified CylLL-S15T in human cellsDepositorInsertCylLL-S15T
UseTagsMultiple tags due to polycistronic expressionExpressionMammalianMutationS15TPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac dual_Human full length _CDK7 (S164D, T170E)_del327-346
Plasmid#217012PurposeExpression of CDK7 in insect cellsDepositorInsertCDK7 (S164D, T170E)_del327-346 (CDK7 Human)
UseTagsTEV cleavable his tagExpressionInsectMutation(S164D, T170E)_del327-346PromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11
Plasmid#174644PurposeOptogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomesDepositorInsertKIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
UseTagsVenus-SSPB and iLID-mCherryExpressionMammalianMutationmmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…PromoterChicken beta-actinAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His6 TEV cloning vector with BioBrick polycistronic restriction sites (11B)
Plasmid#48295DepositorTypeEmpty backboneUseTagsHis6ExpressionInsectMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His6 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-B)
Plasmid#55219DepositorTypeEmpty backboneUseTagsHis6ExpressionInsectMutationPromoterAvailable sinceJune 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T
Plasmid#225062PurposeExpression and Nuclear-localization of CylM-modified CylLL-S15T in human cellsDepositorInsertCylLL-S15T
UseTagsMultiple tags due to polycistronic expressionExpressionMammalianMutationS15TPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_PML1 or 2-FLAG-YFast-TEV-6xHis-CylLL S15T-P2A-Myc-CylM
Plasmid#225061PurposeExpression and Plasma membrane-localization of CylM-modified CylLL-S15T in human cellsDepositorInsertCylLL-S15T
UseTagsMultiple tags due to polycistronic expressionExpressionMammalianMutationS15TPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (11C)
Plasmid#48296DepositorTypeEmpty backboneUseTagsHis6 and MBPExpressionInsectMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (E44A L47A D49A A50K Q51A; deltaWIPI2)
Plasmid#223777PurposeExpression of recombinant protein for purificationDepositorInsertBNIP3 soluble part (BNIP3 Human)
UseTagsThrombin cleavage-GSTExpressionInsectMutationE44A L47A D49A A50K Q51A; disrupts WIPI2 bindingPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5-FRT-TO-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11
Plasmid#174646PurposeOptogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes. Compatible with Flp-in TREX system.DepositorInsertKIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
UseTagsExpressionMammalianMutationmmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…PromoterCMV (with TetOn)Available sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)
Plasmid#55217DepositorTypeEmpty backboneUseTagsSNAPf and TwinStrepIIExpressionInsectMutationPromoterAvailable sinceJuly 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)
Plasmid#55220DepositorTypeEmpty backboneUseTagsHis6 and MBP, Asn10ExpressionInsectMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)
Plasmid#55216DepositorTypeEmpty backboneUseTagsSNAPf and TwinStrepIIExpressionInsectMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only