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  4. pUC-ODN-hsRPL3-CterTwStrep

    Plasmid
    #208644
    Purpose
    for long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.
    Depositor
    Quanfu Ma
    Insert
    Twin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
    Use
    CRISPR; Ssdna donnor preparation
    Tags
    Twin Strep
    Expression
    Mutation
    Promoter
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208644-plasmid-map-sequence-id-418358

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  15. pAGD335

    Plasmid
    #219499
    Purpose
    pAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoter
    Depositor
    Seth Shipman
    Article
    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.
    Insert
    Eco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219499-plasmid-map-sequence-id-435415

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  20. pAD-GamS

    Plasmid
    #99246
    Purpose
    Expresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.
    Depositor
    Jeff Hasty
    Article
    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102
    Insert
    gamS (with C-terminal 6xHis tag)
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Oct. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99246-plasmid-map-sequence-id-339990

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Showing: 401 - 420 of 2593 results