We narrowed to 25,801 results for: STI

1. pSLPB2B-FKBP12_F36V-SpdCas9-hHDAC4-tagBFP-PGK-Blasticidin

   Plasmid

   #187954

   Purpose

   FKBP12 (F36V mutant) degron-tagged dCas9-hHDAC4 fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   FKBP12_F36V-SpdCas9-hHDAC4-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII

   Plasmid

   #217897

   Purpose

   Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tag

   Depositor

   Brenda Schulman

   Article

   Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

   Inserts

   PSMB1 (PSMB1 Human)
   PSMB2 (PSMB2 Human)
   PSMB3 (PSMB3 Human)
   PSMB4 (PSMB4 Human)
   

   Use

   Tags

   2xSTII

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMGS55 (GFP-ARF16-PB1-MCS-P2A-OsTIR1)

   Plasmid

   #129667

   Purpose

   A repair construct to express GFP-ARF16-PB1-MCS and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   OsARF16-PB1 domain
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. 221-pCAGGS-HA-AID-CTCFcDNA-AID-eGFP-2A-blasticidin

   Plasmid

   #195101

   Purpose

   Vector to introduce human CTCF cDNA tagged with eGFP and two AIDs in the cells. Blasticidin selection (2A-fusion). Auxin-inducible degron system.

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   CTCF (CTCF Human)
   

   Use

   Tags

   AID, HA, and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGS

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2

   Plasmid

   #182350

   Purpose

   Ubiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

   Plasmid

   #187945

   Purpose

   dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   SpdCas9-tagRFPt-P2A-tagBFP
   

   Use

   Synthetic Biology; Piggybac

   Tags

   NLS, NLS + HA-tag, P2A, tagBFP, and tagRFPt

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Padh1-OsTIR1(F74A)-NLS-TADH1-Sp.arg3+/pUC19*

   Plasmid

   #169353

   Purpose

   Expression of OsTIR1(F74A) in S. pombe from adh1 promoter, integrated at arg3-D4 locus

   Depositor

   Tony Carr

   Insert

   OsTIR1(F74A)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   F74A

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX208-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

   Plasmid

   #125231

   Purpose

   For generating SPARK2 NanoLuc-βarrestin2-TEVp lentivirus

   Depositor

   Alice Ting

   Article

   Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

   Insert

   NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEM386 - mosTI unc-119 - NNN - gfp - tbb-2

   Plasmid

   #182345

   Purpose

   GFP fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A

    Plasmid

    #182049

    Purpose

    expression of Sox2 mutants

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S248A/T258A

    Promoter

    CAG

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-SFFV-myc-STIM1-WPRE-UbC-Emerald

    Plasmid

    #225939

    Purpose

    Lentiviral vector plasmid expressing human stromal interaction molecule 1 (STIM1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis, Evan Reid

    Inserts

    STIM1 (STIM1 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-SFFV-myc-STIM2-WPRE-UbC-Emerald

    Plasmid

    #225940

    Purpose

    Lentiviral vector plasmid expressing human stromal interaction molecule 2 (STIM2) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis, Evan Reid

    Inserts

    STIM2 (STIM2 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSAH46 - MosTI [unc-119(partial) | MCS | gfp]

    Plasmid

    #200366

    Purpose

    Cloning vector for mosTI(unc-119, gfp). MCS or Golden Gate.

    Depositor

    Christian Froekjaer-Jensen

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSAH45 - MosTI [unc-119(partial) | MCS | wrmScarlet]

    Plasmid

    #200365

    Purpose

    Cloning vector for mosTI(unc-119, wrmScarlet). MCS or Golden Gate.

    Depositor

    Christian Froekjaer-Jensen

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSEM323 - array co-injection fragment mosTI Pmlc-2_GFP

    Plasmid

    #159836

    Purpose

    Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    array co-injection fragment mosTI Pmlc-2_GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

    Plasmid

    #89873

    Purpose

    expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    bArrestin2-TevC-P2A-TdTomato
    

    Use

    AAV

    Tags

    TdTomato

    Expression

    Mammalian

    Mutation

    R173H in TdTomato (Please see depositor comments …

    Promoter

    Human synapsin

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSEM414 - mosTI unc-119 Peft-3 - gfp(nls) - tbb-2

    Plasmid

    #182357

    Purpose

    Positive control for mosTI(unc-119). unc-119 rescue and ubiquitous nuclear GFP expression after mosTI insertion.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Peft-3

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSEM396 - mosTI unc-119 - NNN - operon - gfp - tbb-2

    Plasmid

    #182353

    Purpose

    GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PCDNA3-muOR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

    Plasmid

    #113611

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    mu opioid receptor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)

    Plasmid

    #38262

    Depositor

    Robert Lefkowitz

    Article

    Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

    Insert

    β-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    Flag and PIP5K Iα core kinase domain (residues 18…

    Expression

    Mammalian

    Mutation

    Arg 233, Arg237, and Lys251 converted to Glutamin…

    Promoter

    CMV

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only