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PKC gamma dNPS

Plasmid

#21237

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC gamma (Prkcg Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

delta 1-28

Promoter

Available since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
AP1muA-mStayGold-polyA-Hygro HR

Plasmid

#229679

Purpose

Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells

Depositor

Francesca Bottanelli

Article

Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

Insert

genomic sequence AP1M1 locus (AP1M1 Human)

Use

CRISPR

Tags

monomeric StayGold

Expression

Mutation

Promoter

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-BRAF-REG-Halo

Plasmid

#194771

Purpose

Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag.

Depositor

Deborah Morrison

Article

Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

Insert

BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV51

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHJ346

Plasmid

#20678

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Moesin (MSN Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed Lys 253, 254, 262, 263 to Asn

Promoter

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pHJ386

Plasmid

#20679

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Moesin (MSN Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed Lys 253, 254, 262, 263 to Asn; Changed Th…

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
SLC9A3R1_PDZ_2

Plasmid

#103933

Purpose

Protein expression and purification of SLC9A3R1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

SLC9A3R2 (NHERF2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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PDZK1_PDZ_1

Plasmid

#103927

Purpose

Protein expression and purification of PDZK1 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDZK1 (PDZK1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
SLC9A3R2_PDZ_2

Plasmid

#103926

Purpose

Protein expression and purification of NHERF2 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

SLC9A3R2 (NHERF2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PDZK1_PDZ_4

Plasmid

#103945

Purpose

Protein expression and purification of PDZK1 PDZ4 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDZK1 (PDZK1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TJP2_PDZ_3

Plasmid

#103949

Purpose

Protein expression and purification of X104 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TJP2 (TJP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TJP2_PDZ_2

Plasmid

#103955

Purpose

Protein expression and purification of X104 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TJP2 (TJP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MPDZ_PDZ_7

Plasmid

#103891

Purpose

Protein expression and purification of Multiple PDZ protein PDZ7 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MPDZ (MPDZ Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MPDZ_PDZ_13

Plasmid

#103897

Purpose

Protein expression and purification of Multiple PDZ protein PDZ13 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MPDZ (MPDZ Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MPDZ_PDZ_3

Plasmid

#103887

Purpose

Protein expression and purification of Multiple PDZ protein PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MPDZ (MPDZ Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTrcHis_VectorA_short_speGpromoter_SAT1 (human)

Plasmid

#220477

Purpose

heterologous expression of SSAT1 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Use

Tags

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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SNTA1_PDZ_1

Plasmid

#103928

Purpose

Protein expression and purification of STNA1 PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

SNTA1 (SNTA1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MPP1_PDZ_1

Plasmid

#103931

Purpose

Protein expression and purification of MPP1 PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MPP1 (MPP1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PDZK1_PDZ_2

Plasmid

#103943

Purpose

Protein expression and purification of PDZK1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDZK1 (PDZK1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PDZK1_PDZ_3

Plasmid

#103944

Purpose

Protein expression and purification of PDZK1 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDZK1 (PDZK1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
INADL_PDZ_5

Plasmid

#103905

Purpose

Protein expression and purification of INADL PDZ5 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

INADL (PATJ Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge