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pJH2972
Plasmid#175285PurposePmyo-3 GFP::DAF-16a unc-54 3' UTR C.elegans muscle expression of isoforms a of DAF-16DepositorInsertDAF-16a (daf-16 Nematode)
UseTagsGFPExpressionWormMutationPromoterPmyo-3Available sinceNov. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJK_kanR_proJK1_PAD
Plasmid#60630PurposeConstitutively expresses PAD (phenolic acid decarboxylase)DepositorInsertPhenolic acid decarboxylase padC (padC )
UseTagsExpressionBacterialMutationK146YPromoterproJK1Available sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS306:Met3p-mCherry-Tub1
Plasmid#37554DepositorInsertMet3p-mCherry-Tub1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS303:VC
Plasmid#37556DepositorInsertVenus C-term (aa 155-238)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.2-GW/EmGFP-miR-SUMO23
Plasmid#31073DepositorUseRNAiTagsEmGFPExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET21c-IF3mt
Plasmid#31078DepositorInsertMature form human mitochondrial translational initiation factor 3 (MTIF3 Human)
UseTagsHisExpressionBacterialMutationIncludes coding sequence for the mature form. Ami…PromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Casp3 C163A-myc
Plasmid#11814DepositorInsertCaspase 3 catalytic mutant (CASP3 Human)
UseTagsMycExpressionMammalianMutationC163APromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGLO-GFP-3UAG
Plasmid#82501PurposeExpresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153DepositorInsertGreen Fluorescence Protein with 3 UAG codons
UseTagsExpressionBacterialMutationAdded an UAG codon at the amino acid position 3, …PromoterAraBADAvailable sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
ATX3-77Q
Plasmid#184249PurposeRecombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertAtaxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Synthetic, Human)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationCodon optimization for protein expression in BL2…PromoterT7 promoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATX3-77Q-R388G
Plasmid#184251PurposeRecombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track and point mutation R388G (ATXN3 Synthetic, Human)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationC-terminal codon optimization for protein expres…PromoterT7 promoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ2
Plasmid#197415PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-2 σ2
UseTagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationencodes R42G substitution, contains silent substi…PromoterADH1 and MET25Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ef1a-DIO-eGCaMP(2+)
Plasmid#201150PurposeAAV virus production for Cre recombinase dependent expression of eGCaMP(2+)DepositorInserteGCaMP_2+
UseAAV, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ef1a-DIO-eGCaMP(plus)
Plasmid#201149PurposeAAV virus production for Cre recombinase dependent expression of eGCaMP(plus)DepositorInserteGCaMP+
UseAAV, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC8b-XI3up
Plasmid#176170Purposeyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-3DepositorInsert5' homology for integration site XI-3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-XI5dw
Plasmid#176173Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-5DepositorInsert3' homology for integration site XI-5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-XII5dw
Plasmid#176177Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-5DepositorInsert3' homology for integration site XII-5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-X2dw
Plasmid#176163Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-2DepositorInsert3' homology for integration site X-2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-XI1dw
Plasmid#176167Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-1DepositorInsert3' homology for integration site XI-1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC7-XI2dw
Plasmid#176169Purposeyeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-2DepositorInsert3' homology for integration site XI-2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only
