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Plasmid#120997PurposeMoClo golden gate assembly CD part (phage lysis protein; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.DepositorInsertX174E
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C11m
Plasmid#120959PurposeMoClo golden gate assembly CD part for Citrine (modified YFP; variant of https://www.fpbase.org/protein/citrine/) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertCitrine
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C5m
Plasmid#120957PurposeMoClo golden gate assembly CD part for mRFP (monomeric red fluorescent protein; see https://doi.org/10.1073/pnas.082243699 and https://www.fpbase.org/protein/mrfp1/).DepositorInsertmRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C115m
Plasmid#121014PurposeMoClo golden gate assembly CD part for TP901 integrase (Integrase from lactococcal phage TP901). Please see Supplemental Documents for annotated Genbank file.DepositorInsertTP901 integrase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U22m
Plasmid#121025PurposeMoClo golden gate assembly BC part (high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD6
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C32m
Plasmid#120967PurposeMoClo golden gate assembly CD part for CinR (Quorum-sensing receptor for 3OC14). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohCD carb CinR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C36m
Plasmid#120970PurposeMoClo golden gate assembly CD part for RhlI (quorum-sensing synthase for 3OC14). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohCD carb RhlI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C40m
Plasmid#120973PurposeMoClo golden gate assembly CD part for blank part (random 50bp sequence) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertBlank (Random 50bp sequence)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C59m
Plasmid#120976PurposeMoClo golden gate assembly CD part for mScarlet (RFP variant; see https://www.fpbase.org/protein/mscarlet/). Please see Supplemental Documents for annotated Genbank file.DepositorInsertmScarlet
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C13m
Plasmid#120960PurposeMoClo golden gate assembly CD part for Cerulean (modified CFP; variant of https://www.fpbase.org/protein/cerulean/) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertCerulean
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P1m
Plasmid#120934PurposeMoClo golden gate assembly AB part plasmid for J23101 (strength 1791x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson); AB overhangs.DepositorInsertJ23101
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICH47742:FCP:Cas9YFP
Plasmid#85986PurposeGolden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoterDepositorInsertCas9
UseCRISPR; Golden gate assemblyTagsSV40 NLS, YFPExpressionMutationPromoterFCPAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICH47732:FCP:NAT
Plasmid#85984PurposeGolden Gate Level 1 cassette encoding a Nat selectable marker under the FCP promoterDepositorInsertNAT
UseGolden gate assemblyTagsExpressionMutationPromoterFCPAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
(B) G418 marker (R) (SBE 137)
Plasmid#195011Purposegeneticin (G418) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOSDepositorInsertpXYL+G418+tNOS
UseTagsExpressionBacterialMutationPromoterpXYLAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMulti_K_ccdB
Plasmid#223852PurposeGEC-acceptor for 4G-cloning, designed for expression in E. coli (Kanamyicin resistance)DepositorTypeEmpty backboneUseGolden-gate acceptorTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMulti_S_ccdB
Plasmid#223853PurposeGEC-acceptor for 4G-cloning, designed for expression in E. coli (Streptomycin resistance)DepositorTypeEmpty backboneUseGolden-gate acceptorTagsExpressionBacterialMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMulti_A_ccdB
Plasmid#223851PurposeGEC-acceptor for 4G-cloning, designed for expression in E. coli (Ampicillin resistance)DepositorTypeEmpty backboneUseGolden-gate acceptorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
P18m
Plasmid#120941PurposeMoClo golden gate assembly AB part for T7 promoter (strong, T7 polymerase-dependent constitutive promoter; use with C95m). Please see Supplemental Documents for annotated Genbank file.DepositorInsertT7
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C31m
Plasmid#120966PurposeMoClo golden gate assembly CD part for Bxb1 integrase. See DOI: 10.1007/s00438-006-0129-5. Please see Supplemental Documents for annotated Genbank file.DepositorInsertbxb1 integrase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P9m
Plasmid#120937PurposeMoClo golden gate assembly AB part for pOR1-OR2 (lambda cI-repressable strong promoter) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertPr-OR1-OR2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only