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We narrowed to 554 results for: mak

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Showing: 401 - 420 of 554 results

  1. pICE-HA-CtIP-siR-N289A-H290A

    Plasmid
    #82032
    Purpose
    Plasmid for constitutive or doxy-inducible expression of N289A+H290A mutant of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Insert
    CtIP (RBBP8 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Endonuclease-dead mutant: N289A+H290A CtIP; Mutat…
    Promoter
    CMV-tet
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82032-plasmid-map-sequence-id-155382

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  3. Sema3f(S, +)-AP-His

    Plasmid
    #72023
    Purpose
    Expresses the Sema3F protein (truncated at cleavage site P1; ie, short and contains no deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Publication
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sema3f (Sema3f Mouse)
    Tags
    AP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72023-plasmid-map-sequence-id-131209

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  4. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid
    #163643
    Purpose
    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Inserts
    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    Use
    Lentiviral
    Tags
    Luc2-P2A-tdTomato-T2A
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    EFS and pU6
    Available since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    163643-plasmid-map-sequence-id-325021

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  6. MBC2-null

    Plasmid
    #162749
    Purpose
    MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genes
    Depositor
    Maki Nakayama
    Publication
    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11
    Insert
    murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
    Use
    Retroviral
    Expression
    Mammalian
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162749-plasmid-map-sequence-id-320949

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  7. MBC1-null

    Plasmid
    #162748
    Purpose
    MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes
    Depositor
    Maki Nakayama
    Publication
    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11
    Insert
    murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
    Use
    Retroviral
    Expression
    Mammalian
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162748-plasmid-map-sequence-id-320948

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  8. pcDNA3-Ro1

    Plasmid
    #24504
    Depositor
    Bruce Conklin
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RASSL Ro1 (OPRK1 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    mutations in extracellular loop 2, making it a RA…
    Available since
    June 25, 2010
    Availability
    Academic Institutions and Nonprofits only

  9. pNS3

    Plasmid
    #131770
    Purpose
    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites
    Depositor
    Jared Toettcher
    Publication
    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d
    Insert
    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
    Use
    Integration into δ-sites
    Expression
    Yeast
    Mutation
    E490G to make Cry2 into Cry2Olig
    Promoter
    PADH1
    Available since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    131770-plasmid-map-sequence-id-257191

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  18. A3Bi-ctd E255A -Cas9n-UGI-NLS

    Plasmid
    #109429
    Purpose
    Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Publication
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109429-plasmid-map-sequence-id-212934

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  19. pCW-GemininΔDbox-HA-Puro

    Plasmid
    #193770
    Purpose
    Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Publication
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    GemininΔDbox-HA (GMNN Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    Dbox removed (R23A, L26A). Codons switched at siR…
    Promoter
    TRE
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193770-plasmid-map-sequence-id-380213

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Showing: 401 - 420 of 554 results