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pCMV5B-TFGbeta receptor II K277R

Plasmid

#11762

Depositor

Joan Massague, Jeff Wrana

Publication

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

TGFbeta receptor II (TGFBR2 Human)

Tags

His

Expression

Mammalian

Mutation

K277R: kinase dead

Promoter

CMV

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pET28a-MH6-EsCas13d

Plasmid

#108303

Purpose

Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

Depositor

Arbor Biotechnologies

Publication

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

EsCas13d

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Lck-mTurquoise2

Plasmid

#98822

Purpose

In vivo visualization of the plasma membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Lck

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pRK5 FLAG wildtype, catalytic active lipin 1

Plasmid

#32005

Depositor

David Sabatini

Publication

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 (Lpin1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV, SP6

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pTRE-Fast-FT

Plasmid

#31913

Depositor

Vladislav Verkhusha

Publication

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Fast-FT

Expression

Mammalian

Mutation

M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

Promoter

PTight Tet-responsive PminCMVd

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3 Flag RbBP5

Plasmid

#15550

Depositor

Kai Ge

Publication

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

RbBP5 (RBBP5 Human)

Tags

Flag

Expression

Mammalian

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pTfR-PAmCherry1

Plasmid

#31948

Depositor

Vladislav Verkhusha

Publication

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Tags

transferrin receptor (TfR)

Expression

Mammalian

Mutation

E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

Promoter

CMV

Available since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3.1/Ndi-1/myc-His

Plasmid

#127503

Purpose

Expresses myc-tagged yeast Ndi-1 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Publication

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

Ndi-1 (NDI1 Budding Yeast)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP13

Plasmid

#141266

Purpose

Gateway-compatible Entry vector, with insert of NSP13 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP13 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pET24b-APEX2

Plasmid

#111702

Purpose

For IPTG-inducible expression of APEX2 in E. coli

Depositor

Jessica Seeliger

Publication

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Tags

6xHis and V5

Expression

Bacterial

Promoter

T7 promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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K44A dynamin 1 pEGFP

Plasmid

#34681

Depositor

Sandra Schmid

Publication

Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

Insert

dynamin 1ba (DNM1L Human)

Tags

EGFP

Expression

Mammalian

Mutation

K44A

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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SaKKH-ABE8e

Plasmid

#138502

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSaCas9 (KKH)

Expression

Mammalian

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-C-ABE8e

Plasmid

#185667

Purpose

Expresses eNme2-C-ABE8e in mammalian cells

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-C

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pACYCT2

Plasmid

#45799

Depositor

Luc Ponchon

Publication

Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

Type

Empty backbone

Expression

Bacterial

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MT-cdc20

Plasmid

#11593

Depositor

Marc Kirschner

Publication

Pfleger et al Genes Dev. 2001 Sep 15. 15(18):2396-407.

Insert

cdc20 (CDC20 Human)

Tags

myc

Expression

Mammalian

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV-3XFLAG-hINO80

Plasmid

#44149

Depositor

Joan Conaway

Publication

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

hINO80 full length (INO80 Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Lamp1-mTurquoise2

Plasmid

#98828

Purpose

In vivo visualization of lysosomes (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Lamp1

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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HA Sec13 pRK5

Plasmid

#46332

Depositor

David Sabatini

Publication

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Sec13 (SEC13 Human)

Tags

HA

Expression

Mammalian

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pORTMAGE-4

Plasmid

#72679

Purpose

Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.

Depositor

Csaba Pál

Publication

Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

Insert

mutL E32K

Tags

none

Expression

Bacterial

Mutation

E32K mutation conferring dominant mutator phenoty…

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEYFP-Sec24C

Plasmid

#66608

Purpose

YFP fusion of Sec24C

Depositor

David Stephens

Publication

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec24C (SEC24C Human)

Expression

Mammalian

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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