19,640 results

1. pCINeoFlag ALIX

   Plasmid

   #89859

   Purpose

   Expresses full length Flag-tagged ALIX in mammalian cells; internal ID WISP03-308

   Depositor

   Wesley Sundquist

   Article

   Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

   Insert

   ALIX (PDCD6IP Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-dCas9

   Plasmid

   #47106

   Purpose

   Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Flag and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGreenPuro-shRNA-Stx3S-C4

   Plasmid

   #99742

   Purpose

   3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.

   Depositor

   Thomas Weimbs

   Article

   Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

   Insert

   shRNA against Stx3S
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. P307-FBP_6

   Plasmid

   #139697

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   various

   Available Since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pECFP18aaEYFP

   Plasmid

   #109330

   Purpose

   ECFP-EYFP FRET positive control

   Depositor

   Gabriele Kaminski Schierle

   Article

   Kaminski et al Methods Mol Biol. 2014;1076:445-54. doi:

   Insert

   ECFP-EYFP FRET positive control
   

   Expression

   Mammalian

   Mutation

   ECFP linked to EYFP with 18 aa linker GLRSRAQASNS…

   Promoter

   CMV

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFPC1-EPSIN 1

   Plasmid

   #22228

   Depositor

   Pietro De Camilli

   Article

   Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

   Insert

   Epsin 1 (Epn1 Rat)
   

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   please see depositor comment below

   Available Since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. MEK1-GFP

   Plasmid

   #14746

   Depositor

   Rony Seger

   Article

   Yao et al Oncogene. 2001 Nov 15. 20(52):7588-96.

   Insert

   MEK1 (MAP2K1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV human IRS-1 CAAX

   Plasmid

   #11238

   Depositor

   Ronald Kahn

   Article

   Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

   Insert

   IRS-1 (IRS1 Human)
   

   Tags

   CAAX

   Expression

   Mammalian

   Mutation

   C-terminal prenylation motif makes IRS-1 localize…

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pBB535

   Plasmid

   #27392

   Depositor

   Bernd Bukau

   Article

   de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

   Insert

   dnaK, dnaJ (dnaJ, dnaK E. coli)
   

   Expression

   Bacterial

   Available Since

   March 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. Proinsulin-NanoLuc in pLX304

    Plasmid

    #62057

    Purpose

    Luminescent reporter of insulin secretion

    Depositor

    David Altshuler

    Article

    Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

    Insert

    Proinsulin-NanoLuc (Ins2 Mouse, Synthetic)
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Mutation

    Inserted NanoLuc into C-peptide of mouse Ins2 cod…

    Promoter

    CMV

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC-BETAipi

    Plasmid

    #53277

    Purpose

    Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

    Insert

    idi
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    endogenous promoter

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-hERG1a

    Plasmid

    #53052

    Purpose

    Expresses alpha subunit of hERG1a K channel in mammalian cells

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Raptor_1 shRNA

    Plasmid

    #1857

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

    Plasmid

    #174665

    Purpose

    Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter

    Depositor

    Eli Bar

    Inserts

    LUC2
    mCherry:T2A:Hygro
    

    Use

    Lentiviral and Luciferase

    Tags

    mCherry and hygromycin dual reporter gene linked …

    Expression

    Mammalian

    Promoter

    Human cytomegalovirus immediate early enhancer/pr…

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hsyn-GRAB_ATP1.0

    Plasmid

    #167577

    Purpose

    Express the ATP sensor GRAB_ATP1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP sensor GRAB_ATP1.0 (P2RY1 Human)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. SnailHA_pcDNA3

    Plasmid

    #31697

    Depositor

    Paul Wade

    Article

    Kajita et al Mol Cell Biol. 2004 Sep . 24(17):7559-66

    Insert

    Snail (SNAI1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330A-FBL/PITCh

    Plasmid

    #63671

    Purpose

    Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q109

    Plasmid

    #111730

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q109 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFPC2-Gephyrin P1

    Plasmid

    #68815

    Purpose

    expression of fluorescent tagged Geph in mammalian cells

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silent mutations on internal EcoRI site. Gephyri…

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. Human MAC-1 alpha

    Plasmid

    #8631

    Depositor

    Timothy Springer

    Article

    Corbi et al J Biol Chem 1988 Sep 5;263(25):12403-11.

    Insert

    MAC-1 alpha (ITGAM Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only