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We narrowed to 25,138 results for: crispr

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Showing: 4181 - 4200 of 25138 results

  9. pET-28b-T7-henAsCas12a(E174R/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1885)

    Plasmid
    #114072
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a(E174R/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, and K548R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114072-plasmid-map-sequence-id-220522

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  10. pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

    Plasmid
    #123363
    Purpose
    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.
    Depositor
    Sidi Chen
    Publication
    Chow et al Nat Methods. 2019 May;16(5):405-408. doi
    Insert
    EGFP-NLS
    Use
    CRISPR, Lentiviral, and Luciferase
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123363-plasmid-map-sequence-id-240469

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  11. pLentiGuide

    Plasmid
    #117986
    Purpose
    pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.
    Depositor
    Paul Khavari
    Publication
    Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31
    Insert
    sgRNA backbone (F+E)
    Use
    Lentiviral
    Available since
    Feb. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    117986-plasmid-map-sequence-id-235371

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  13. pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187949
    Purpose
    SMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SMASh-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187949-plasmid-map-sequence-id-373500

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  14. Target-AID (pRZ762)

    Plasmid
    #131300
    Purpose
    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131300-plasmid-map-sequence-id-254651

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  19. pCMMP-MCS-IRES-eGFP-P2A-iCas9

    Plasmid
    #178533
    Purpose
    For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells
    Depositor
    Ryoji Yao
    Publication
    Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iCaspase9 (CASP9 Human)
    Expression
    Mammalian
    Mutation
    FKBP12 F36 to V Truncated Caspase9 (135-416; lack…
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    178533-plasmid-map-sequence-id-351918

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Showing: 4181 - 4200 of 25138 results