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We narrowed to 4,942 results for: puc

Showing: 4181 - 4200 of 4942 results

  1. pGS1022

    Plasmid
    #59576
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Twin-Strep tag optimized for PCR amplification followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Expression
    Mutation
    Promoter
    None
    Available since
    Aug. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    59576-plasmid-map-sequence-id-92983

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  3. ATP1A1_G8_Dual_sgRNA

    Plasmid
    #178104
    Purpose
    Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G8 sgRNA + user-specified sgRNA
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178104-plasmid-map-sequence-id-350193

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  5. attB‐mCh­‐Poly(A)-­PuroR

    Plasmid
    #65529
    Purpose
    PuroR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.
    Depositor
    Sebastian Bultmann
    Article
    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv
    Insert
    attB-mCherry
    Use
    Mouse Targeting; Bxb1
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    65529-plasmid-map-sequence-id-111228

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  6. kiCAP-AAV-M.Mus.1

    Plasmid
    #196681
    Purpose
    Rep/Cap plasmid for the production of M.Mus.1, an AAV capsid with CNS tropism in mice.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    WVLPSGG insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196681-plasmid-map-sequence-id-389742

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  7. kiCAP-AAV-MDV1B

    Plasmid
    #196680
    Purpose
    Rep/Cap plasmid for the production of MDV1B, an AAV capsid with CNS tropism in mice.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    KTVGTVY insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    196680-plasmid-map-sequence-id-386956

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  9. pEasyG2_mic

    Plasmid
    #184916
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184916-plasmid-map-sequence-id-371408

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  11. pGfa28-nLac

    Plasmid
    #53130
    Purpose
    Expression of LacZ driven by partial human gfa2 promoter, expression is localized to a subset of astrocytes in mice.
    Depositor
    Michael Brenner
    Article
    Lee et al Glia. 2006 May;53(7):677-87.
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    contains the A and B regions (bp -1757 to -1489) …
    Promoter
    human Gfa2
    Available since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    53130-plasmid-map-sequence-id-82730

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  13. ATP1A1_G8_MTOR_Nick_Exon-53_Dual_sgRNA

    Plasmid
    #178107
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G8 + MTOR nick exon-53 sgRNAs
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178107-plasmid-map-sequence-id-350194

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  14. pRP(Exp)-CMV> ORF_924bp (Azgp1):dTomato:IRES:Puro

    Plasmid
    #110831
    Purpose
    Expresses both zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter, where the fluorescent dT reporter is fused with the ZAG protein.
    Depositor
    Ande Satyanarayana
    Article
    Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:
    Insert
    Azgp1 (Azgp1 Mouse)
    Use
    Non-viral gene expression vector
    Tags
    dTomato
    Expression
    Mutation
    Promoter
    CMV
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    110831-plasmid-map-sequence-id-214111

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  16. ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA

    Plasmid
    #173214
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA
    Use
    CRISPR; Prime editing (pe3)
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173214-plasmid-map-sequence-id-339507

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  19. pYI2206

    Plasmid
    #228348
    Purpose
    Constitutive rPhlTA mutant expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    phlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    P5S, S6P, K86T, F109L, Q117R, E143K
    Promoter
    KpGAPDH promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228348-plasmid-map-sequence-id-457096

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  20. pMT1026

    Plasmid
    #228299
    Purpose
    Constitutive expression of PhlTA mutant with weak promoter and terminator
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    phlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    E41G
    Promoter
    KpYPT1 promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228299-plasmid-map-sequence-id-457081

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Showing: 4181 - 4200 of 4942 results