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p5081 pcDNA4c hBrd4 (1134-1223)

Plasmid

#14457

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1134-1223) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 1134-1223

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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ps226

Plasmid

#133557

Purpose

RNAi construct for knock-down of CDKN1A (Cip1, p21)

Depositor

David Livingston

Article

Chan et al Genes Dev. 2005 Jan 15;19(2):196-201. do

Insert

CDKN1A (CDKN1A Human)

Use

Retroviral

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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mKO-Mito-7

Plasmid

#57848

Purpose

Localization: Mitochondria, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Mito (COX8A Human)

Tags

mKO

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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GST-GAR

Plasmid

#34697

Depositor

Steven Clarke

Article

Frankel et al J Biol Chem. 2000 Oct 20;275(42):32974-8

Insert

Fibrillarin (FBL Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 1-145 of human fibrillarin with K2E and …

Promoter

tac

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only
mTFP1-H3-23

Plasmid

#55489

Purpose

Localization: Nucleus/Histones, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

H3 (H3C2 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCI-FLAG-mRRP6

Plasmid

#60045

Purpose

Mammalian expression of FLAG-tagged mouse RRP6

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

RRP6 (Exosc10 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only
pMA7

Plasmid

#79967

Purpose

TM-MAGE strain

Depositor

Morten Sommer

Article

Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

Inserts

Lambda Red recombinase beta subunit
DNA adenine methylase

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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NG0750 pCI-TPI-PTC48-4H

Plasmid

#65803

Purpose

NMD reporter mRNA

Depositor

Niels Gehring

Article

Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

Insert

TPI (TPI1 Human)

Tags

4H (4 copies of short beta-globin 3'UTR for …

Expression

Mammalian

Mutation

premature stop codon at 48

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-f:PGC1(delta CTD)

Plasmid

#1030

Depositor

Bruce Spiegelman

Article

Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

Insert

PGC1a (Ppargc1a Mouse)

Tags

Flag

Expression

Mammalian

Mutation

delta CTD

Available Since

Oct. 4, 2005

Availability

Academic Institutions and Nonprofits only

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QRFP-Tango

Plasmid

#66491

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

QRFP (QRFPR Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-Lin28A-mCCHC

Plasmid

#51372

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

Lin28A (LIN28A Human)

Tags

FLAG

Expression

Mammalian

Mutation

CCHC domain mutant (H147A, H169A)

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
FLAG-β-arrestin2 RRK/Q

Plasmid

#38261

Depositor

Robert Lefkowitz

Article

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Insert

β-arrestin2 (Arrb2 Rat)

Tags

Flag

Expression

Mammalian

Mutation

Arg233, Arg237, and Lys251 converted to Glutamines

Promoter

CMV

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
pFUW-TetO-SUB1

Plasmid

#178440

Purpose

doxycycline-inducible expression of Human SUB1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

SUB1 (SUB1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHisAlix deltaPRD

Plasmid

#42577

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

delta-PRD ALIX (residues 1-702) (PDCD6IP Human)

Tags

HIS-TEV

Expression

Bacterial

Mutation

silent mutation A1617G (numbering in ALIX gene)

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pPPC001

Plasmid

#171138

Purpose

For integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Promoter

Sp.pCas9, BBa_J23107

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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HP21-TPI-PTC1

Plasmid

#131043

Purpose

Expresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid) with a 21 nt hairpin in 5'UTR

Depositor

Marvin Tanenbaum

Article

Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

Insert

TPI-PTC1 (TPI1 Human)

Expression

Mammalian

Mutation

PTC1

Promoter

Pcmv

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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mWasabi-Mito-7

Plasmid

#56508

Purpose

Localization: Mitochondria, Excitation: 493, Emission: 509

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Mito (COX8A Human)

Tags

mWasabi

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
RnBarr1-mTurquoise2

Plasmid

#137789

Purpose

Visualization of beta-arrestin1

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin 1 (Arrb1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pPbuCas13b-gRNA-NT

Plasmid

#184568

Purpose

Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PbuCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Constitutive expression of single-spacer CRISPR array witha nontargeting spacer for PbuCas13b in bacteria.

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pet23a_RBPMS_FL

Plasmid

#59392

Purpose

Plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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