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pENTR-FLAG-HAND1-T2A-mTagBFP2

Plasmid

#223192

Purpose

Entry vector containing FLAG-HAND1 with a T2A-mTagBFP2 reporter (attL flanked)

Depositor

Matthew Birket

Article

Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

Insert

HAND1 (HAND1 Human)

Use

Promoterless entry vector for gateway cloning

Tags

FLAG and T2A-mTagBFP2

Expression

Bacterial

Promoter

None

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-LC3A

Plasmid

#223726

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

LC3A (MAP1LC3A Human)

Tags

GST

Expression

Bacterial

Mutation

aa 2-120 only, with Glycine exposed

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGIG-shCdkn1a(#4)

Plasmid

#228913

Purpose

Knockdown of Cdkn1a in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

shCdkn1a (Cdkn1a Mouse)

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

Plasmid

#200793

Purpose

Mammalian expression of CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al iScience. 2026 Feb 2;29(3):114875. doi:

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

1-914; Δ505-610

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTag4C_SP-DRD2-V2R-NTEV-tcs-GV-2xHA

Plasmid

#227103

Purpose

DRD2 receptor for split TEV assay

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

DRD2-NTEV (DRD2 Human)

Tags

2xHA

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2374

Plasmid

#225631

Purpose

High Copy number plasmid containing Ple391 (DRD2 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

Ple391 (DRD2 Human)

Use

Unspecified

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-ZNF706-Brd 460

Plasmid

#219365

Purpose

Transcription factor ZNF706 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

ZNF706 (ZNF706 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-SP140-Brd 405

Plasmid

#219328

Purpose

Transcription factor SP140 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

SP140 (SP140 Human)

Use

Lentiviral

Mutation

Codon optimized

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Gse1-exon minimal CMV pCDNA5

Plasmid

#212056

Purpose

LR vector for integration of Gse1-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Gse1-exon (Gse1 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Gse1+exon minimal CMV pCDNA5

Plasmid

#212057

Purpose

LR vector for integration of Gse1+ex(108nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Gse1+ex(108nt) (Gse1 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_DCAF15_ZNF333

Plasmid

#205801

Purpose

Express mEGFP-tagged fusion protein, DCAF15_ZNF333 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

DCAF15_ZNF333 (DCAF15, ZNF333 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ37, 115-133, 204-217 PPM1H

Plasmid

#207344

Purpose

Mammalian expression of HA-Δ37, 115-133, 204-217 PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ37, Δ115-133, Δ204-217

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1064

Plasmid

#143917

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NFATC1 (NFATC1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1063

Plasmid

#143916

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NFATC1 (NFATC1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1060

Plasmid

#143915

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NFATC1 (NFATC1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1058

Plasmid

#143913

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NFATC1 (NFATC1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3487

Plasmid

#144963

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NFATC1 (NFATC1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-HAND1

Plasmid

#170689

Purpose

doxycycline-inducible overexpression of HAND1

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

HAND1 (HAND1 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2510

Plasmid

#144032

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NAT10 (NAT10 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF0590

Plasmid

#143419

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FOXO6 (FOXO6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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