We narrowed to 24,612 results for: Nes

1. pCfB2909(XII-5 MarkerFree)

   Plasmid

   #73281

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV EFIa DIO LMO11 (GeNL_SS-ChRmine)

   Plasmid

   #219426

   Purpose

   Cre-dependent expression of luciferase opsin fusion protein (luminopsin) for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

   Insert

   mNeonGreen, ISN-IE, ChRmine
   

   Use

   AAV

   Tags

   Gaussia luciferase signal sequence

   Expression

   Mutation

   Promoter

   EF-1alpha

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pC-HYG-YR

   Plasmid

   #61765

   Purpose

   Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).

   Depositor

   Ken Haynes

   Article

   Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

   Inserts

   Hygromycin
   2 micron origin or replication and URA3 gene for S. cerevisiae
   

   Use

   Ternary vector for agrobacterium mediated transfo…

   Tags

   Expression

   Mutation

   Promoter

   trpC promoter

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-OC-IRES-BSD

   Plasmid

   #53118

   Purpose

   To create stable cell clones with high-level expression of Cas9 and OCT1

   Depositor

   Wensheng Wei

   Article

   Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Inserts

   OCT1 (POU2F1 Human)
   Cas9
   

   Use

   Lentiviral

   Tags

   IRES-BSD, NLS, and P2A

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available Since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pBabe RFP1-Smad2 neo

   Plasmid

   #58491

   Purpose

   mammalian expression of mRFP1-tagged mouse Smad2

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   Smad2 (Smad2 Mouse)
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

6. KIF5C(1-560)-2xmCherry-EF(C)

   Plasmid

   #61664

   Purpose

   Tandem mCherry-labeled, truncated kinesin-1 fused to EF Hand linker

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   KIF5C(1-560)-2xmCherry-EF(C) (Kif5c Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBBK03 pCAS-Tyr-[gRNA: BsaI GFP dropout] (HygR,AmpR)

   Plasmid

   #178987

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBBK04 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitHygR,AmpR)

   Plasmid

   #178988

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitHygromycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pB-Ric8b-Integration

   Plasmid

   #129457

   Purpose

   piggyBac transposon vector that Inducibly (Tet-On) expresses Ric8b

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   Ric8b (Ric8b Mouse)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE (Tet-On)

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLIK 3xFLAG-BCAS2 zeo

    Plasmid

    #192319

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-BCAS2

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    BCAS2 (BCAS2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBM019

    Plasmid

    #128179

    Purpose

    Construct for generating stable Cas9-expressing Toxoplasma gondii

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

    Inserts

    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3X FLAG and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. NEK7_HUMAN_D0

    Plasmid

    #79735

    Purpose

    This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    NEK7 (NEK7 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pLX302 GDF11-V5 puro

    Plasmid

    #83097

    Purpose

    Lentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    GDF11 (GDF11 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

14. pLX302-MKP5-V5 puro

    Plasmid

    #87770

    Purpose

    Expresses V5 tagged human MAPK phosphatase 5 (MKP5/DUSP10)

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLX304 GDF11-V5 blast

    Plasmid

    #83098

    Purpose

    Lentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    GDF11 (GDF11 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-mCREB

    Plasmid

    #68551

    Purpose

    AAV expression of mCREB

    Depositor

    Eric Nestler

    Article

    Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

    Insert

    mCREB
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S133A

    Promoter

    CMV

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

    Plasmid

    #114111

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100

    Insert

    Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. UAS-u(S)Cas9

    Plasmid

    #127383

    Purpose

    Expresses Cas9 at high level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(S)-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAST

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. YE1-BE4max-CP1028

    Plasmid

    #138160

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R126E; within Cas9 (CP1028…

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKMD111e - intein-Cas9(S219-G521R)

    Plasmid

    #64192

    Purpose

    Expresses intein-Cas9(S219-G521R) in mammalian cells

    Depositor

    David Liu

    Article

    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

    Insert

    mammalian codon-optimized S. pyogenes intein-Cas9(S219-G521R) - NLS - 3x FLAG
    

    Use

    CRISPR

    Tags

    NLS - 3x FLAG (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge