Skip to main content
Addgene
Showing: 4221 - 4240 of 77185 results

  1. pMA_Int

    Plasmid
    #110096
    Purpose
    E. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Insert
    mycophage integrase
    Use
    Required for transformation of attb integrating v…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110096-plasmid-map-sequence-id-211299

    Enlarge

  2. ZJOM100

    Plasmid
    #133662
    Purpose
    Integration of MNN9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-MNN9-mTagBFP2 (MNN9 Budding Yeast)
    Use
    Tags
    mTagBFP2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133662-plasmid-map-sequence-id-267533

    Enlarge

  3. ZJOM103

    Plasmid
    #133664
    Purpose
    Integration of SEC7-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-SEC7-mTagBFP2 (SEC7 Budding Yeast)
    Use
    Tags
    mTagBFP2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    133664-plasmid-map-sequence-id-292147

    Enlarge

  4. pMM0641

    Plasmid
    #129006
    Purpose
    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129006-plasmid-map-sequence-id-259445

    Enlarge

  5. ZJOM96

    Plasmid
    #133663
    Purpose
    Integration of SEC26-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-SEC26-mTagBFP2 (SEC26 Budding Yeast)
    Use
    Tags
    mTagBFP2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133663-plasmid-map-sequence-id-265883

    Enlarge

  6. ZJOM135

    Plasmid
    #133659
    Purpose
    Integration of SEC63-2mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-SEC63-2mTagBFP2 (SEC63 Budding Yeast)
    Use
    Tags
    mTagBFP2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133659-plasmid-map-sequence-id-265905

    Enlarge

  8. ZJOM141

    Plasmid
    #133669
    Purpose
    Integration of COX9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-COX9-mTagBFP2 (COX9 Budding Yeast)
    Use
    Tags
    mTagBFP2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    133669-plasmid-map-sequence-id-265880

    Enlarge

  9. ZJOM108

    Plasmid
    #133665
    Purpose
    Integration of mTagBFP2-Tlg1 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-mTagBFP2-TLG1 (TLG1 Budding Yeast)
    Use
    Tags
    mTagBFP2
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133665-plasmid-map-sequence-id-292111

    Enlarge

  10. ZJOM12

    Plasmid
    #133638
    Purpose
    Integration of GFP-TLG2 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    pCUP1-GFP-Tlg2 (TLG2 Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133638-plasmid-map-sequence-id-269549

    Enlarge

  12. pMM0647

    Plasmid
    #129008
    Purpose
    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    KanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129008-plasmid-map-sequence-id-259450

    Enlarge

  13. pAGN33

    Plasmid
    #131105
    Purpose
    An integrative plasmid able to introduce a second copy of the pip gene in the chromosome of our conditional mutants
    Depositor
    Riccardo Manganelli
    Article
    Boldrin et al Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1
    Insert
    P-furA102tetO-pip
    Use
    Tags
    Expression
    Bacterial
    Mutation
    the furA102 promoter was mutated with the inserti…
    Promoter
    Available since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131105-plasmid-map-sequence-id-262707

    Enlarge

  14. pCRISPEY-Z3-Hyg-ADE2

    Plasmid
    #232107
    Purpose
    Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    Z3
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232107-plasmid-map-sequence-id-462308

    Enlarge

  15. AA428

    Plasmid
    #216055
    Purpose
    Fragmid fragment: (C' terminus) NpNLS; 3xHA; c-mycNLS; c-mycNLS
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    NpNLS_v1.1; 3xHA_v1.1; c-mycNLS_v1.2; c-mycNLS_v1.3
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216055-plasmid-map-sequence-id-431407

    Enlarge

  16. pL0-NbPT5bPro

    Plasmid
    #159416
    Purpose
    Nicotiana benthamiana PT5b promoter sequence (1068 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Insert
    NbPT5b Promoter
    Use
    Puc19-derived
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    159416-plasmid-map-sequence-id-323164

    Enlarge

  18. pMM0649

    Plasmid
    #129010
    Purpose
    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporter
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    129010-plasmid-map-sequence-id-270698

    Enlarge

  19. pMM0655

    Plasmid
    #129016
    Purpose
    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporter
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    129016-plasmid-map-sequence-id-261247

    Enlarge

  20. pMM0654

    Plasmid
    #129015
    Purpose
    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporter
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129015-plasmid-map-sequence-id-259463

    Enlarge

Showing: 4221 - 4240 of 77185 results