We narrowed to 2,485 results for: organization Addgene

1. pEGFP N1 AURKA 1-30

   Plasmid

   #157756

   Purpose

   Expression of AURKA Nter (aa1-30):GFP in mammalian cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The first 30 amino acids of AURKA were cloned

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-VRER-P2A-GFP-PGK-Puro

   Plasmid

   #110862

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRER
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-HF1-P2A-GFP-PGK-Puro

   Plasmid

   #110863

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-FAB(PX-LD)

   Plasmid

   #214422

   Purpose

   Detection of peroxisome-lipid droplet contact site

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   PMP34-FKBP-V5-RspAN_IRES_CFAST-20nm-Halo-6xHp NC (SLC25A17 Human)
   

   Use

   Lentiviral

   Tags

   CFAST-Halo and FKBP-V5-RspAN

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. W45A mutant of H3K9me3 biosensor

   Plasmid

   #120808

   Purpose

   FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

   Promoter

   CMV

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. K9L mutant of H3K9me3 biosensor

   Plasmid

   #120807

   Purpose

   FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   lysine 9 is mutated to leucine 9 on histone H3

   Promoter

   CMV

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204214

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DNA binding domain (delDBD)

   Promoter

   TetON

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-human Erbb2-IRES-GFP

   Plasmid

   #91888

   Purpose

   Retroviral expression of human Erbb2

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Erbb2 (ERBB2 Human)
   

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   MMLV gag

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-WT-WLS-HA-IRES-GFP

   Plasmid

   #178061

   Purpose

   Expression of WLS-HA protein (WT)

   Depositor

   Joseph Gleeson

   Article

   Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

   Insert

   WLS (WLS Human)
   

   Use

   Tags

   HA and IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-mouse Ezh2 Y637S-IRES-GFP

    Plasmid

    #91887

    Purpose

    Retroviral expression of mouse Ezh2 with Y637S

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    Ezh2 (Ezh2 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Y637S

    Promoter

    MMLV gag

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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