We narrowed to 17,703 results for: SHE

1. PAS_HeSAHS4

   Plasmid

   #215648

   Purpose

   Expresses SUMO-tagged HeSAHS4 protein from tardigrade H. exemplaris in E. coli

   Depositor

   Pamela Silver

   Insert

   SAHS4
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PAS_RvSAHS4

   Plasmid

   #215646

   Purpose

   Expresses SUMO-tagged RvSAHS4 protein from tardigrade R. varieornatus in E. coli

   Depositor

   Pamela Silver

   Insert

   SAHS4
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX458-tRNA-SpCas9

   Plasmid

   #195183

   Purpose

   Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.

   Depositor

   Stephanie Roessler

   Insert

   Cas9
   

   Use

   CRISPR

   Promoter

   tRNA-Gln

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7CFE1_EVD68_VP12A_C107R

   Plasmid

   #212982

   Purpose

   Encodes EV-D68 MO/14/18947 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 VP12A C107R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Mutation

   changed cysteine 107 from 2A to Arginine

   Promoter

   T7

   Available Since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7CFE1_EVD68_3ABC_C147R

   Plasmid

   #212978

   Purpose

   Encodes EV-D68 MO/14/18947 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 3ABC C147R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Mutation

   changed Cysteine 147 in 3C to Arginine

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7CFE1_EVD68_3ABCD_C147R

   Plasmid

   #212980

   Purpose

   Encodes EV-D68 MO/14/18947 3ABCD with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 3ABCD C147R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Mutation

   changed cysteine 147 from 3C to arginine

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7CFE1_EVD68_VP12A_N84T

   Plasmid

   #212983

   Purpose

   Encodes EV-D68 MO/14/18947 VP12A with telaprevir resistance mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 VP12A N84T
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Mutation

   changed Asparagine 84 from 2A to Threonine

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-Flag-RBM24-1~93

   Plasmid

   #208633

   Purpose

   for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93

   Depositor

   Quanfu Ma

   Insert

   human RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)
   

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   only aa 1~93, other residues not included

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-RPL3-Twstrep

   Plasmid

   #208623

   Purpose

   for E. coli expression of C-ter Twin Strep tagged human RPL3 (ENST00000216146)

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) (RPL3 Human)
   

   Tags

   6xHis and Twin Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAMW-Wnk

    Plasmid

    #203978

    Purpose

    Myc6 Wnk under actin promoter for cell culture

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Tags

    6xMyc

    Expression

    Insect

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. XBB.1.5+R403K

    Plasmid

    #208586

    Purpose

    Mammalian expression vector for expressing XBB.1.5+R403K SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation

    Depositor

    Ben Murrell

    Insert

    SARS-CoV-2 variant XBB.1.5+R403K spike (S )
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. XBB.1.5+F456L

    Plasmid

    #208585

    Purpose

    Mammalian expression vector for expressing XBB.1.5+F456L SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation

    Depositor

    Ben Murrell

    Insert

    SARS-CoV-2 variant XBB.1.5+F456L spike (S )
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. XBB.1.5+K478R

    Plasmid

    #208587

    Purpose

    Mammalian expression vector for expressing XBB.1.5+K478R SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation

    Depositor

    Ben Murrell

    Insert

    SARS-CoV-2 variant XBB.1.5+K478R spike (S )
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-Flag-hS1pr1-S5A_Neo

    Plasmid

    #203710

    Purpose

    Overexpression of FLAG-S1PR1-S5A mutant.

    Depositor

    Timothy Hla

    Insert

    Flag-hS1PR1-S5A (S1PR1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S5A

    Promoter

    CMV

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pALuWBB

    Plasmid

    #183202

    Purpose

    dsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNA

    Depositor

    Mitch McVey

    Insert

    w+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
    

    Use

    CRISPR; Recombines at attp landing site in fly ge…

    Expression

    Insect

    Promoter

    U6-3

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PVALB mAb_BCN14.1.1A2_HC_Rat IgG2a

    Plasmid

    #182894

    Purpose

    Mammalian expression of PVALB rat mAb HC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) heavy chain (PVALB Rat)
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PVALB mAb_BCN14.1.1A2_LC_Rat Ig Kappa

    Plasmid

    #182895

    Purpose

    Mammalian expression of PVALB rat mAb LC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) light chain (PVALB Rat)
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. TRE3GV-somBiPOLES-mKate2

    Plasmid

    #192579

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of tTA-positive neurons

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Promoter

    TRE3GV

    Available Since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRE3GV-somBiPOLES-mCerulean

    Plasmid

    #192578

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of tetTag-positive neurons

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Promoter

    TRE3GV

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. CaMKII-somBiPOLES-eGFP

    Plasmid

    #192583

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Promoter

    CaMKIIa(0.4)

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLenti-PGK-NXPH4 (neo)

    Plasmid

    #192078

    Purpose

    Lentivirus for expression of NXPH4

    Depositor

    Roland Friedel

    Insert

    NXPH4 (NXPH4 Human)
    

    Use

    Lentiviral

    Promoter

    hPGK

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSpCas9_mouse_Adar1_ccC

    Plasmid

    #158121

    Purpose

    deletion mAdar1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (Adar Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Plasmid

    #170789

    Purpose

    Expresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Depositor

    Kalle Gehring

    Insert

    Toxoplasma gondii PRL
    

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lacI

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pB6-Rosa26-cre_dre_reporter_1_2

    Plasmid

    #185966

    Purpose

    B6J Rosa26-cre dre reporter 1.2 allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    sfGFP, tdTomato
    

    Use

    Cre/Lox and Mouse Targeting; Dre/rox

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pB6-Rosa26-cre_dre_reporter_1_1b

    Plasmid

    #185965

    Purpose

    B6J Rosa26-cre dre reporter 1.1b allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    sfGFP, tdTomato
    

    Use

    Cre/Lox and Mouse Targeting; Dre/rox

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pB6-Rosa26-cre_dre_reporter_1_1a

    Plasmid

    #185964

    Purpose

    B6J Rosa26-cre dre reporter 1.1a allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    sfGFP, tdTomato
    

    Use

    Cre/Lox and Mouse Targeting; Dre/rox

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pB6-Rosa26-cre_dre_reporter_1_1

    Plasmid

    #185963

    Purpose

    B6J Rosa26-cre dre reporter 1.1 allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    EGFP, tdTomato
    

    Use

    Cre/Lox and Mouse Targeting; Dre/rox

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector

    Plasmid

    #182346

    Purpose

    PacI-flanked CAG-LSL expression cassette with a FNF for positive selection

    Depositor

    Mario Capecchi

    Insert

    tdTomato
    

    Use

    Mouse Targeting; Flp / frt

    Expression

    Mammalian

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. R26-CAG-rox-FNF-bpA-3pA-rox-tdTomato

    Plasmid

    #180154

    Purpose

    tdTomato dre reporter with Rosa26 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    tdTomato
    

    Use

    Mouse Targeting; Dre/rox

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SDCBPA-c021

    Plasmid

    #175485

    Purpose

    Protein expression in bacterial cells. PDZ1 and PDZ2 domains (truncated at C-terminus), G111-F275.

    Depositor

    Opher Gileadi

    Insert

    SDCBP (SDCBP Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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