We narrowed to 27,280 results for: Tat

1. pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)

   Plasmid

   #216531

   Purpose

   Targeting vector for CRISPRd generation

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   ABI-dCas9-mCherry
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-FLAG-APEX2-LGALS3

   Plasmid

   #175756

   Purpose

   Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS3

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-FKBP-PIP4K2A

   Plasmid

   #202732

   Purpose

   expresses recruitable fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2A (PIP4K2A, FKBP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-HA-DNM2-R465W

   Plasmid

   #155262

   Purpose

   Mammalian expression of rat DNM2 fused to HA

   Depositor

   David Rubinsztein

   Article

   Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

   Insert

   dynamin 2 (Dnm2 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Arginine 465 to Tryptophan

   Promoter

   Available since

   May 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWZL Blast Snail ER

   Plasmid

   #18798

   Depositor

   Bob Weinberg

   Article

   Mani et al Cell. 2008 May 16. 133(4):704-15.

   Insert

   snail (SNAI1 Human)
   

   Use

   Retroviral

   Tags

   ER hormone binding domain

   Expression

   Mammalian

   Mutation

   G525R mutation in the ER. The presence of the G52…

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRES2Cx32WTEGFP

   Plasmid

   #78150

   Purpose

   Mammalian Expression of Connexin 32 and EGFP from a bicistronic mRNA

   Depositor

   Mario Bortolozzi

   Article

   Carrer et al Hum Mol Genet. 2017 Oct 25. pii: 4565496

   Insert

   Connexin 32 (GJB1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-K-GECO1

   Plasmid

   #105865

   Purpose

   Mammalian cell expression plasmid for red fluorescent Ca2+ indicator K-GECO1

   Depositor

   Robert Campbell

   Article

   Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

   Insert

   K-GECO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEP4-FLAG-CCR5

   Plasmid

   #98949

   Purpose

   Mammalian expression plasmid for N-terminal FLAG-tagged human CCR5

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CCR5 (CCR5 Human)
   

   Use

   Tags

   FLAG (dykdddd) epitope tag and Signal/leader sequ…

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Tag2B-E2F1

   Plasmid

   #202520

   Purpose

   Expressing human E2F1 protein with FLAG-tag

   Depositor

   Ming Chen

   Article

   Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

   Insert

   E2F1 (E2F1 Human)
   

   Use

   Tags

   FLAG-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. alpha catenin conformation sensor

    Plasmid

    #71709

    Purpose

    This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    alpha-catenin FRET Sensor
    

    Use

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-MCS-shCHD5 #1

    Plasmid

    #68876

    Purpose

    CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-MCS-shCHD5 #2

    Plasmid

    #68877

    Purpose

    CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

13. human gamma-catenin pcDNA3

    Plasmid

    #16827

    Depositor

    Eric Fearon

    Article

    Caca et al Cell Growth Differ. 1999 Jun . 10(6):369

    Insert

    gamma-catenin (JUP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. ETS1_pet28a

    Plasmid

    #131663

    Purpose

    Bacterial expression of ETS gene ETS1

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETS1 (ETS1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDH-IFNA-neo

    Plasmid

    #225710

    Purpose

    The pCDH-IFNA-neo plasmid is designed for the overexpression of human IFNα in mammalian cells. This plasmid was utilized to engineer A549 cells for stable IFNα overexpression.

    Depositor

    Eun Hee Han

    Article

    Min et al Commun Biol. 2024 Sep 30;7(1):1222. doi:

    Insert

    IFNA (IFNA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCPH2A.X_S139A-GS3

    Plasmid

    #204749

    Purpose

    Expression of Cas9 and human H2AX with the S139A mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX S139A (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the S139A mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. HiBiT-FZD7

    Plasmid

    #195845

    Purpose

    Expresses human FZD7 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD7 (FZD7 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWZL Blast Twist ER

    Plasmid

    #18799

    Depositor

    Bob Weinberg

    Article

    Mani et al Cell. 2008 May 16. 133(4):704-15.

    Insert

    twist (Twist1 Mouse)
    

    Use

    Retroviral

    Tags

    ER hormone binding domain

    Expression

    Mammalian

    Mutation

    G525R mutation in the ER. The presence of the G52…

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SOX9-T2A-H2B-EGFP repair template

    Plasmid

    #167972

    Purpose

    SOX9 reporter repair template

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    SRY-Box Transcription Factor 9 (SOX9 Human)
    

    Use

    CRISPR; Donor template

    Tags

    H2B-EGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA 3.2/V5-DEST SNAP29-V5

    Plasmid

    #69821

    Purpose

    Expresses SNAP29-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Synaptosomal-associated protein 29 (SNAP29 Human)
    

    Use

    Retroviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 20XUAS IVS ChrimsonR mCherry_tr

    Plasmid

    #111546

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

    Depositor

    Vivek Jayaraman

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR_mCherry_trafficked
    

    Use

    Tags

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV-FLEX-PKIalpha-IRES-nls-mRuby2

    Plasmid

    #63059

    Purpose

    Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    PKIalpha (Pkia Mouse, Synthetic)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ETV4_pet28a

    Plasmid

    #131645

    Purpose

    Bacterial expression of ETS gene ETV4

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETV4 (ETV4 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mCherry-FKBP-PIP4K2B

    Plasmid

    #202734

    Purpose

    expresses recruitable fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    mCherry:FKBP1A (3-108):[GGSA]4GG:PIP4K2B (PIP4K2B, FKBP1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNAZeo(-) LAMP2C

    Plasmid

    #89342

    Purpose

    Expresses human LAMP-2C in mammalian cells

    Depositor

    Janice Blum

    Article

    Perez et al J Immunol. 2016 Mar 15;196(6):2457-65. d

    Insert

    Lysosomal-associated membrane protein 2C (LAMP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-ATF6-(S1P-)

    Plasmid

    #32956

    Purpose

    For visualization of ATF6 in mammalian cells with a mutation in ATF6 that blocks cleavage by protease S1P. Activated protein will remain in the Golgi.

    Depositor

    Ron Prywes

    Article

    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

    Insert

    ATF6 (ATF6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S1Protease site mutation: R415A/R416A

    Promoter

    CMV

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAM5188

    Plasmid

    #120087

    Purpose

    Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.

    Depositor

    Susan Golden

    Article

    Chen et al Microbiology. 2016 Dec;162(12):2029-2041

    Insert

    pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMVS102_P3-GFP-NATMX6

    Plasmid

    #99048

    Purpose

    EGFP reporter with six binding sites for the Zif268 DNA binding domain

    Depositor

    Barak Cohen

    Article

    Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1

    Inserts

    McIsaac 2014 P3 promoter
    eGFP
    clonNAT resistance
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    GAL1 promoter with 4 GAL4 sites removed and repla…

    Promoter

    McIsaac 2014 P3 promoter

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLPC-FLAG.Mm.EPC2

    Plasmid

    #184649

    Purpose

    Retroviral expression of mouse EPC2

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    Epc2 (Epc2 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLPC-FLAG.Mm.EPC1

    Plasmid

    #184648

    Purpose

    Retroviral expression of mouse EPC1

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    EPC1 (Epc1 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. LUC01 (L/Sec/P)

    Plasmid

    #112144

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cysteine 258 mutated to selenocysteine (U)

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. p2.1 (pAGL2A)

    Plasmid

    #27563

    Depositor

    Gregg Semenza

    Article

    Semenza et al J Biol Chem. 1996 Dec 20. 271(51):32529-

    Insert

    ENO1 promoter (ENO1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

33. pC-FLAG-ZCD2

    Plasmid

    #79274

    Purpose

    Mammalian expression of ZCD2 with a C-terminal FLAG tag

    Depositor

    Rita Shiang

    Article

    Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

    Insert

    ZCD2 (CISD2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

34. FLI1_pet28a

    Plasmid

    #131641

    Purpose

    Bacterial expression of ETS gene FLI1

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    FLI1 (FLI1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAG-cofilin-S3A

    Plasmid

    #78296

    Purpose

    Mammalian expression of mouse cofilin S3A (nonphosphorylatable form) fused to EGFP

    Depositor

    Michael Frotscher

    Article

    Chai et al Development. 2016 Mar 15;143(6):1029-40.

    Insert

    cofilin 1 (Cfl1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S3A

    Promoter

    CAG

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEGFP-Sec24D

    Plasmid

    #32678

    Depositor

    Henry Lester

    Article

    Richards et al J Biol Chem. 2011 Sep 9;286(36):31241-9.

    Insert

    Sec24D (SEC24D Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-Tag2B-E2F3

    Plasmid

    #202522

    Purpose

    Expressing human E2F3 protein with FLAG-tag

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    E2F3 (E2F3 Human)
    

    Use

    Tags

    FLAG-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pECE M2-SH3PXD2A WT

    Plasmid

    #69813

    Purpose

    Expresses M2-SH3PXD2A in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mcherry p62 delta UBA silent

    Plasmid

    #187984

    Purpose

    mcherry p62 lacking UBA domain (aa389-434) but it has the silent mutations to be resistant to sip62. Internal reference: SMC572

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    p62 (NUP62 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Lacking UBA domain (aa389-434) but it has the sil…

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TALED_Left-mouse-ATP6-1397N.

    Plasmid

    #212951

    Purpose

    Expresses TALED_Left-mouse-ATP6-1397N in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

    Insert

    TALED_Left-mouse-ATP6-1397N (ATP6 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge