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pD649-HAsp-SIRPD-COMP5AP-AviTag-9xHis

Plasmid

#157128

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIRPD (SIRPD Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTT3-SP-6xHis-TIE1(FL)-FLAG

Plasmid

#157621

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TIE1 (TIE1 Human)

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On shYAP1-6

Plasmid

#193667

Purpose

Tet inducible knockdown of YAP1

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On shYAP1-2

Plasmid

#193665

Purpose

Tet inducible knockdown of YAP1

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shYAP1-2

Plasmid

#193693

Purpose

Constitutive lentiviral expression of YAP1 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Cdipt-V5

Plasmid

#175144

Purpose

Lentiviral expression of mouse Cdipt-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Cdipt (Cdipt Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTD73

Plasmid

#172897

Purpose

Intestinal promoter ges-1P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi4348 site on chromosome I

Depositor

David Reiner

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

TIR-1 (TIR1 Mustard Weed)

Use

CRISPR

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pTD81

Plasmid

#172902

Purpose

Hypodermal promoter col-10P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi5605 site on chromosome II

Depositor

David Reiner

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

TIR-1 (TIR1 Mustard Weed)

Use

CRISPR

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-BAEBL (I1134V/P1135L/Y1136V/F1137V)

Plasmid

#154875

Purpose

Expresses N-terminally GFP-tagged Pf-BAEBL P5-P2 VLVV variant (coding for residues 1041-1210) from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfBAEBL (PF3D7_0506900 P. falciparum)

Tags

GFP and signal peptide (residues 1 to 32) from TG…

Expression

Mammalian

Mutation

I1134V, P1135L, Y1136V, F1137V; insert codes for …

Promoter

CMV

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-KAT14-547-782

Plasmid

#118376

Purpose

Gateway compatible donor vector with KAT14-547-782 truncation.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

KAT14 (KAT14 Human)

Use

Donor-plasmid

Mutation

Truncation (aa 547-782)

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pJet-CMV-CD52-bglpA

Plasmid

#89704

Purpose

Human CD52 expression plasmid with short polyA

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

CMV-CD52-bglpA (CD52 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR83

Plasmid

#106035

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR83; Opto-GPR83; D1)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR83 (GPR83 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR87

Plasmid

#106038

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR87; Opto-GPR87; D4)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR87 (GPR87 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-KAT14-1-546

Plasmid

#118377

Purpose

Gateway compatible donor vector with KAT14-1-546 truncation.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

KAT14 (KAT14 Human)

Use

Donor-plasmid

Mutation

Truncation (aa 1-546)

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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W250R EPHA3 pcDNA3.1

Plasmid

#102744

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Tags

myc-6xhis

Expression

Mammalian

Mutation

W250R (TGG > AGG)

Promoter

CMV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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D678E EPHA3 pcDNA3.1

Plasmid

#102750

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Tags

myc-6xhis

Expression

Mammalian

Mutation

D678E (GAC > GAa)

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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T660K EPHA3 pcDNA3.1

Plasmid

#102749

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Tags

myc-6xhis

Expression

Mammalian

Mutation

T660K (ACA > AAA)

Promoter

CMV

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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S449F EPHA3 pcDNA3.1

Plasmid

#102748

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Tags

myc-6xhis

Expression

Mammalian

Mutation

S449F (TCC > TTC)

Promoter

CMV

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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A435S EPHA3 pcDNA3.1

Plasmid

#102747

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Tags

myc-6xhis

Expression

Mammalian

Mutation

A435S (GCT > TCT)

Promoter

CMV

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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N379K EPHA3 pcDNA3.1

Plasmid

#102745

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Tags

myc-6xhis

Expression

Mammalian

Mutation

N379K (AAT > AAG)

Promoter

CMV

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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