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p23-mNeongreen-DDX21 DAT

Plasmid

#179229

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

DDX21 (DDX21 Human)

Use

Tags

mNeongreen

Expression

Mammalian

Mutation

G234D, K235A

Promoter

EF-1alpha

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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SB-CAG-mCherry-P2A-EIF3D-DD-IP

Plasmid

#236770

Purpose

A sleeping beauty-based vector containing CAG promoter-driven mCherry-P2A-EIF3D S528D/S529D-IRES-Pac.

Depositor

Kazutoshi Takahashi

Article

Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

Insert

EIF3D (EIF3D Human)

Use

Sleeping beauty

Tags

Expression

Mammalian

Mutation

changed Serine 528 to Aspartic Acid and Serine 52…

Promoter

CAG promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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SB-CAG-mCherry-P2A-EIF3D-NN-IP

Plasmid

#236771

Purpose

A sleeping beauty-based vector containing CAG promoter-driven mCherry-P2A-EIF3D S528N/S529N-IRES-Pac.

Depositor

Kazutoshi Takahashi

Article

Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

Insert

EIF3D (EIF3D Human)

Use

Sleeping beauty

Tags

Expression

Mammalian

Mutation

changed Serine 528 to Asparagine and Serine 529 t…

Promoter

CAG promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pJet-CMV-IARS1[W435C]-GFP

Plasmid

#212323

Purpose

EGFP-tagged IARS1 Trp435Cys

Depositor

Sabine Fuchs

Article

Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

Insert

IARS (IARS1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Trp435cys

Promoter

CMV

Available since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-GMIP(trunc N1)

Plasmid

#221278

Purpose

Expression of GMIP N-terminal truncation 1 (16-84) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

GMIP N-terminal truncation 1 (16-84) (Gmip Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-MAVS(pLxIS) E433A

Plasmid

#221258

Purpose

Expression of MAVS pLxIS (393-441) mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS pLxIS (393-441) (Mavs Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Expression

Mutation

E433A

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-MAVS(GMIP pLxIS)

Plasmid

#221316

Purpose

Expression of MAVS chimera with GMIP pLxIS domain in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS chimera with GMIP pLxIS domain (Mavs Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-GMIP(trunc C1)

Plasmid

#221279

Purpose

Expression of GMIP C-terminal truncation 1 (1-64) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

GMIP C-terminal truncation 1 (1-64) (Gmip Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-GMIP(trunc C2)

Plasmid

#221280

Purpose

Expression of GMIP C-terminal truncation 2 (1-44) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

GMIP C-terminal truncation 2 (1-44) (Gmip Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-MAVS(trunc Cterm)

Plasmid

#221259

Purpose

Expression of MAVS truncation (393-431) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS truncation (393-431) (Mavs Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-STING(pLxIS) S365A

Plasmid

#221241

Purpose

Expression of mutant STING CTT in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

STING CTT (Sting1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

S356A

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-STING(pLxIS) L373A

Plasmid

#221242

Purpose

Expression of mutant STING CTT in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

STING CTT (Sting1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

L373A

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p23-mRuby3-DDX21 HGV

Plasmid

#179233

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

DDX21 (DDX21 Human)

Use

Tags

mRuby3

Expression

Mammalian

Mutation

D338H, E339G

Promoter

EF-1alpha

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLV hCgrrf1-V5

Plasmid

#175129

Purpose

Lentiviral expression of human CGRRF1-V5

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

CGRRF1 (CGRRF1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLV mCgrrf1-V5

Plasmid

#175128

Purpose

Lentiviral expression of mouse Cgrrf1-V5

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Cgrrf1 (Cgrrf1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-N3FLAG-TAF12(50-130)

Plasmid

#105595

Purpose

retrovirally express mouse TAF12 HFD with 3*FLAG tag at N terminal, GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 (AA 50-130)- histone fold domain-HFD (Taf12 Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

truncation fragments containing aa (50-130), and …

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pET12a-HAL

Plasmid

#47097

Depositor

Marina Ramirez-Alvarado

Insert

HAL

Use

Tags

ompT

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe4-RfA

Plasmid

#186375

Purpose

Destination vector for the expression of a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Type

Empty backbone

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pDLucBiDi

Plasmid

#219562

Purpose

Dual luciferase plasmid with Renilla luciferase constitutively expressed and firefly luciferase downstream of MCS without a start codon

Depositor

Pasha Baranov

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40 and T7

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET12a-JOH1

Plasmid

#47098

Depositor

Marina Ramirez-Alvarado

Insert

JOH1 (IGKV3-20 Human)

Use

Tags

ompT

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only