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pML346_VPS35-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227891

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

VPS35 homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML530_EEA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227892

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

EEA1 homology arms with mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML348_ATG101-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227900

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

ATG101 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML460_MAP1LC3B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227901

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

MAP1LC3B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML349_PEX3-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227902

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

PEX3 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227904

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

SEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227905

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

COPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMB1-spect-PthrC3_8-eGFP

Plasmid

#107411

Purpose

This plasmid contain promoter PthrC3_8, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

Depositor

Kang Zhou

Article

Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

Insert

eGFP

Expression

Bacterial

Promoter

PthrC3_8

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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aMHC-eGFP-Rex-Neo

Plasmid

#21229

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

alpha myosin heavy chain promoter

Use

Lentiviral

Tags

eGFP

Available Since

June 30, 2009

Availability

Academic Institutions and Nonprofits only

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AICSDP-166:CDH1-mEGFP

Plasmid

#193921

Purpose

Homology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1

Depositor

Allen Institute for Cell Science

Insert

cadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)

Use

AAV and CRISPR; Donor template

Tags

linker-mEGFP

Mutation

homology arms contain point mutations to disrupt …

Available Since

Dec. 22, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pIRES2-eGFP-Ofd1-Myc

Plasmid

#24560

Depositor

Jeremy Reiter

Article

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Insert

Ofd1 (Ofd1 Mouse)

Tags

Myc and eGFP

Expression

Mammalian

Available Since

May 20, 2010

Availability

Academic Institutions and Nonprofits only

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pZ P (cHS4)X4 OCT4p eGFP SV40pA v2

Plasmid

#115518

Purpose

Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows OCT4 dependent expression of GFP, and contains cHS4 insulators.

Depositor

Catherine Verfaillie

Article

Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

Insert

eGFP

Use

AAV; Donor plasmid for recombinase-mediated casse…

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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AICSDP-079:DCP1A-mEGFP

Plasmid

#193922

Purpose

Homology arms and mEGFP-SRA for N-terminus tagging of human DCP1A

Depositor

Allen Institute for Cell Science

Insert

DCP1A Homology Arms with mEGFP-SRA (DCP1A Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Mutation

homology arms contain point mutations to disrupt …

Available Since

Dec. 22, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBITE-EGFRvIII-F265-OKT3

Plasmid

#190679

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F265 clone)

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F265-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCMMP-dnPERK-IRES-eGFP

Plasmid

#36954

Depositor

Bill Sugden

Article

Lee et al Blood. 2008 Feb 15;111(4):2280-9. Epub 2

Insert

dnPERK (EIF2AK3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

deletion of aa592-1061 to create dominant negative

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_NES-Kinprola_PKA-mEGFP_WRPE-SV40

Plasmid

#233366

Purpose

hSyn1 driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA-mEGFP

Use

AAV

Tags

NES and mEGFP

Promoter

hSyn1

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-FLuc-K21-Rluc_AH

Plasmid

#148847

Purpose

Mammalian Expression of K21-Rluc

Depositor

Elisa Izaurralde

Insert

K21-Rluc

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-U6-UL29-egfp-U3-UL8

Plasmid

#166694

Purpose

To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (EGFP version).

Depositor

Yujia Cai

Article

Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

Insert

UL8 and UL29 gRNA, EGFP (UL8, UL29 HSV-1)

Use

Lentiviral

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP

Plasmid

#194682

Purpose

CMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_04-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_mEGFP

Plasmid

#187073

Purpose

Mitochondria surface expression of dHaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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