We narrowed to 18,335 results for: met

1. pBAD mApple

   Plasmid

   #91772

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   monomeric Apple
   

   Use

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Mutation

   R97K, E152S, A180S, V207T compared to mApple (FP …

   Promoter

   araBAD

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+ cMyc Dam_f_GFPoNLS

   Plasmid

   #85820

   Purpose

   iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   cMyc Dam_f_GFPoNLS
   

   Use

   Tags

   cMyc and oNLS (optimized nuclear localization sig…

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   L122A.

   Promoter

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. mOrange2-Fibrillarin-7

   Plasmid

   #57957

   Purpose

   Localization: Nucleus/Nucleoli, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Fibrillarin (FBL Human)
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   R36Q, S66F

   Promoter

   CMV

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

4. DF-UAS-sspa-GCaMP6f

   Plasmid

   #67569

   Purpose

   Zebrafish expression of a short photoactivatable fluorescent calcium reporter

   Depositor

   Ehud Isacoff

   Article

   Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

   Insert

   sspa-GCaMP6f
   

   Use

   Zebrafish transgenesis (see :a spinal opsin contr…

   Tags

   Expression

   Mutation

   K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

   Promoter

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

5. Emerald-MannII-N-10

   Plasmid

   #54150

   Depositor

   Michael Davidson

   Article

   Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

   Insert

   MannII (Man2a1 Mouse)
   

   Use

   Tags

   Emerald

   Expression

   Mammalian

   Mutation

   aa 1-112 of MannII aa 1-112 of MannII

   Promoter

   CMV

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

6. ArpC4_pLib

   Plasmid

   #173681

   Purpose

   ArpC4 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

   Depositor

   Roberto Dominguez

   Article

   Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

   Insert

   ArpC4 (ARPC4 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PA-mCherry1-ER-5

   Plasmid

   #57174

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Article

   York et al Nat Methods. 2011 Apr;8(4):327-33. doi:

   Insert

   ER (CALR Human)
   

   Use

   Tags

   PA-mCherry1

   Expression

   Mammalian

   Mutation

   ER Signal Peptide

   Promoter

   CMV

   Available Since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

8. Mouse GFP-PRMT7 Wild Type

   Plasmid

   #65056

   Purpose

   Expresses wild type mouse GFP-PRMT7

   Depositor

   Steven Clarke

   Article

   Feng et al J Biol Chem. 2013 Dec 27;288(52):37010-2

   Insert

   PRMT7 (Prmt7 Mouse)
   

   Use

   Bacterial propagation

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pLJC2-GPT2-3xFLAG

   Plasmid

   #163447

   Purpose

   lentiviral overexpression vector for human GPT2

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   GPT2 (GPT2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   See: comments

   Promoter

   CMV

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCardinal-Rab4a-7

    Plasmid

    #56174

    Purpose

    Localization: Ras GTPase, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Rab4a (RAB4A Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. mRuby2-Keratin-17

    Plasmid

    #55900

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. mRuby2-Actin-7

    Plasmid

    #55888

    Purpose

    Localization: Actin, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Actin (ACTB Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. ArpC5_pLib

    Plasmid

    #173682

    Purpose

    ArpC5 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    ArpC5 (ARPC5 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. ArpC1_pLib

    Plasmid

    #173678

    Purpose

    ArpC1 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    ArpC1 (ARPC1B Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAGGS-Flex-Turbo/GFP

    Plasmid

    #197886

    Purpose

    Can be used to generate AAV virus that will express Turbo/GFP in the presence of Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    Turbo/GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2+ oDam_f_GFPoNLS

    Plasmid

    #85819

    Purpose

    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    oDam_f_GFPoNLS
    

    Use

    Tags

    oNLS (optimized nuclear localization signal) C te…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Promoter

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-REX-GECO0.9

    Plasmid

    #61247

    Purpose

    Expresses REX-GECO0.9 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

    Insert

    REX-GECO0.9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Substitutions relative to R-GECO1: P60R, V61W, R6…

    Promoter

    CMV

    Available Since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

18. mRuby2-JUP-C-14

    Plasmid

    #55899

    Purpose

    Localization: Tight Junctions, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    JUP (JUP Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. AB.pCCL.sin.cPPT.U6.miR-10a-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46581

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-10a Decoy (MIR10A, Mir10a Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pLentiCRISPR-v1-sgGPT2_9

    Plasmid

    #163456

    Purpose

    lentiviral vector expressing Cas9 and an sgRNA targeting GPT2

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    sgRNA 9 targeting GPT2 (GPT2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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