We narrowed to 57,759 results for: plasmid
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pPRIME-CMV-GFP-recipient
Plasmid#11657Purpose3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling GFP expressionDepositorTypeEmpty backboneUseLentiviralTagsGFPExpressionMammalianAvailable sinceMarch 1, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPos-Ma-CmR-TAG111
Plasmid#197571PurposePositive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a oriDepositorInsertsChloramphenicol resistance gene - 111TAG
T7 polymerase -TAG
GFPuv
M. alvus Pyl-tRNA(6)
ExpressionBacterialMutation8TAG/112TAG and 111TAGPromoterAraC, T7, cat promoter (constitutive), and lpp (c…Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV1.X1
Plasmid#209780Purposenon-standard AAV2 rep-AAV1-X1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV1-X1 VP1 gene
UseAAVExpressionMammalianPromoterp41Available sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-NIR-GECO2
Plasmid#159603PurposeAAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2DepositorInsertNIR-GECO2
UseAAVExpressionMammalianPromoterCAGAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA
Plasmid#192552PurposeExpresses nuclear localized tdTomato under CAG promoterDepositorHas serviceAAV9 and AAV PHP.eBInserttdTomato
UseAAVTagsNLSMutationdeleted E273 and G274 compared to FPbase ID: PGG5SPromoterCAGAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Tet2 promoter F
Plasmid#63879PurposeLuciferase reporter plasmid used to study transcriptional control of murine Tet2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTet2 Promoter fragment F
UseLuciferaseExpressionMammalianAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTN4
Plasmid#174212PurposeDonor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locusDepositorAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TBG-mem-TurboID
Plasmid#149413PurposeHepatocyte-specific expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorInsertmem-TurboID
UseAAVTagsHA and V5PromoterTBGAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPolI_SARS-CoV-2_NLuc
Plasmid#183953PurposeEncodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoterDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianPromoterMammalian Pol IAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
PE7_AAVS1_knockin_HDR_donor
Plasmid#223001PurposeHDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPE7
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3
Plasmid#106204PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184S
UseAAVMutationGltI: A184S + mRuby3 fusionPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH2B-mNG2(11)
Plasmid#206043PurposePlasmid expressing a H2B-mNG2(11) fusion in mammalian cells for complementation with the mNG2(1-10) fragment.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B gene fused to the mNG2(11) fragment
UseSynthetic BiologyTagsH2B-mNG2(11)ExpressionMammalianPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUGW-Mito-TurboID
Plasmid#200963Purpose3rd gen lentiviral plasmid for expressing mitochondria localized TurboID with EGFPDepositorInsertMito-TurboID
UseLentiviralTagsEGFPExpressionMammalianAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
OaAEP1_C247A
Plasmid#200307PurposeBacterial expression plasmids for production of C247A mutant of asparaginyl endopeptidase 1 from O. affinis (OaAEP1_C247A)DepositorInsertAsparaginyl endopeptidase from Oldenlandia affinis
TagsSmt3ExpressionBacterialMutationCysteine 247 to AlaninePromoterT7Available sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
T4 td upstream intron-PABP
Plasmid#189972PurposePart 1 plasmid to introduce the T4 td upstream intron and PABP spacer into circRNAsDepositorInsertT4 td upstream intron-PABP
UseCloningPromoterN/AAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
iRFP-H2A
Plasmid#158691PurposeThis plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markersDepositorInsertiRFP670
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZsGreen1-cMYC/pLVX-Puromycin
Plasmid#180278PurposePlasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).DepositorAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO-Tet-On-YAP1-shRNA1
Plasmid#176846Purposelentiviral vector for doxycycline-inducible human YAP1 knockdownDepositorInsertYAP1-Sh1 (YAP1 Human)
UseLentiviralAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only
