We narrowed to 60,338 results for: plasmid

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pCiES

Plasmid

#60010

Purpose

Foamy virus helper plasmid expressing Env, driven by a CMV promoter. Ampicillin resistant.

Depositor

David Russell

Publication

Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

Insert

Env (foamy virus)

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_CH60

Plasmid

#111662

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CH60 (HSPD1 Human)

Expression

Mammalian

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBx-Spas-sgRNA-Kan

Plasmid

#105233

Purpose

Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescens

Depositor

Kristala Prather

Publication

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus sgRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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RINS1

Plasmid

#107290

Purpose

Plasmid for the expression of the RINS1 sensor

Depositor

Dmytro Yushchenko

Publication

Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RINS1

Tags

sfGFP

Expression

Mammalian

Promoter

CMV

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTrig

Plasmid

#104576

Purpose

Copy number inducible plasmid. Contains mini-F origin for maintenance and P1 lytic replication machinery under Lac promoter for copy increase.

Depositor

Harris Wang

Publication

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PLac-repL

Use

Synthetic Biology

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAV-U6+27-tCorn

Plasmid

#106233

Purpose

Express tRNA-Corn as a reporter of U6 promoter activity in mammalian cells

Depositor

Samie Jaffrey

Publication

Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194

Insert

tRNA-Corn

Expression

Mammalian

Promoter

U6

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-sgRNA-lib 2.0

Plasmid

#89638

Purpose

sgRNA expression in mammalian cells after Gateway cloning

Depositor

Wensheng Wei

Publication

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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APOBEC3G_pLX307

Plasmid

#98345

Purpose

Lentiviral expression of APOBEC3G

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

APOBEC3G (APOBEC3G Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP

Plasmid

#90015

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP

Depositor

Julien Béthune

Publication

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Ago2
NBirA*-GFP

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_KRAS_WT

Plasmid

#81923

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_MAPK1_WT

Plasmid

#82145

Purpose

Gateway Donor vector containing MAPK1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAPK1 (MAPK1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSkipFlox

Plasmid

#85797

Purpose

P. falciparum plasmid for expression of both diCre fragments from single casette using skip peptide

Depositor

Tobias Spielmann

Publication

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

split Cre recombinase

Use

Cre/Lox; P. falciparum expression

Tags

FKBP, FRB*

Promoter

Pfcrt

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGEX6P-1-Dendra2

Plasmid

#82436

Purpose

Dendra2 expression plasmid

Depositor

Periklis Pantazis

Publication

Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

Insert

Dendra2

Tags

GST tag

Expression

Bacterial

Promoter

tac

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

Plasmid

#73164

Purpose

multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells

Depositor

Bruce Edgar

Publication

Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:

Insert

mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)

Tags

mRFP1, GFP

Expression

Insect

Mutation

Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BPK2797

Plasmid

#72251

Purpose

Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

Depositor

Keith Joung

Publication

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

D1135V, G1218R, R1335Q, and T1337R

Promoter

CMV

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCW-Flag-CtIP-T847E

Plasmid

#71110

Purpose

mammalian expession of Flag-CtlP T847E mutation

Depositor

Daniel Durocher

Publication

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

T847E

Promoter

TRE promoter, Tet ON

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMJH65

Plasmid

#67273

Purpose

Conjugally transferable red recombinogenic plasmid

Depositor

Mark Liles

Publication

Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

Insert

tetA

Use

Red recombinase, temperature sensitive

Expression

Bacterial

Promoter

tetA promoter of plasmid pACYC184 origin

Available since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJW1259

Plasmid

#61251

Purpose

Cas9 plasmid with sgRNA deleted; for use in C. elegans

Depositor

Jordan Ward

Publication

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

Deletion

Use

CRISPR

Expression

Worm

Mutation

Deleted sgRNA(F+E) sequence from pJW1219

Available since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pBS-GGAC-ATGC

Plasmid

#60949

Purpose

Drosophila, backbone for donor plasmid assembly. CRISPR knock-in.

Depositor

Frank Schnorrer

Publication

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Type

Empty backbone

Use

CRISPR

Available since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBI-MCS-EYFP

Plasmid

#58855

Purpose

The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP

Depositor

Joshua Leonard

Publication

Daringer et al ACS Synth Biol. 2014 Mar 11.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

enhanced yellow fluorescent protein

Expression

Mammalian

Promoter

CMV

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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