We narrowed to 6,558 results for: sas;

1. hprtSAGFP

   Plasmid

   #41594

   Purpose

   In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences

   Depositor

   Maria Jasin

   Article

   Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.

   Insert

   SA-GFP reporter
   

   Use

   Mouse Targeting; Dsb repair reporter substrate

   Expression

   Mammalian

   Available Since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. Intron-Tagging-EGFP-Donor

   Plasmid

   #159740

   Purpose

   Contains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   EGFP
   

   Use

   Intron tagging donor

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. R26-EGFP HMEJ donor vector

   Plasmid

   #137927

   Purpose

   ROSA26 knock-in vector with homology-mediated end joining(HMEJ)-based method

   Depositor

   Hiroshi Kiyonari

   Article

   Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

   Insert

   SA-EGFP-bpA with homology arms
   

   Use

   Mouse Targeting

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. R26-H2B-EGFP HR donor vector

   Plasmid

   #137925

   Purpose

   R0SA26 knock-in vector with homologous recombination-based method

   Depositor

   Hiroshi Kiyonari

   Article

   Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

   Insert

   SA-H2B-EGFP-bpA with homology arms
   

   Use

   Mouse Targeting

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. R26-H2B-mCherry HR donor vector

   Plasmid

   #137928

   Purpose

   R0SA26 knock-in vector with homologous recombination-based method

   Depositor

   Hiroshi Kiyonari

   Article

   Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

   Insert

   SA-H2B-mCherry-bpA with homology arms
   

   Use

   Mouse Targeting

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBK1290-AAV-dSaCas9

   Plasmid

   #223139

   Purpose

   AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHJ17

   Plasmid

   #48356

   Purpose

   Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin/Gancyclovir selectable

   Depositor

   George Cross

   Article

   Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

   Insert

   Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-Ty1-TK-loxP-3' ALD UTR
   

   Use

   Cre/Lox; Knockout in t. brucei

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHJ22

   Plasmid

   #48358

   Purpose

   Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Neomycin/Gancyclovir selectable

   Depositor

   George Cross

   Article

   Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

   Insert

   Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-NEO-Ty1-TK-loxP-3' ALD UTR
   

   Use

   Cre/Lox; Knockout in t. brucei

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. R26-EGFP MMEJ donor vector

   Plasmid

   #137926

   Purpose

   R0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based method

   Depositor

   Hiroshi Kiyonari

   Article

   Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

   Insert

   SA-EGFP-bpA with micro-homology arms
   

   Use

   Mouse Targeting

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHJ18

    Plasmid

    #48357

    Purpose

    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin//Gancyclovir selectable

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-Ty1-TK-loxP-3' ALD UTR
    

    Use

    Cre/Lox; Knockout in t. brucei

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV_A2S-8

    Plasmid

    #188654

    Purpose

    AAV vector expressing SaAID-2S and gRNA

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Inserts

    AID2S--nSaCas9-UGI
    Sa-sgRNA
    

    Use

    AAV and CRISPR

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SaCas9

    Promoter

    Scp1 and U6

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHJ41

    Plasmid

    #48360

    Purpose

    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Blasticidin/Gancyclovir selectable

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BSD-Ty1-TK-loxP-3' ALD UTR
    

    Use

    Cre/Lox; Knockout in t. brucei

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHJ42

    Plasmid

    #48359

    Purpose

    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Bleomycin/Gancyclovir selectable

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BLE-Ty1-TK-loxP-3' ALD UTR
    

    Use

    Cre/Lox; Knockout in t. brucei

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHW1133

    Plasmid

    #229869

    Purpose

    Destination RMCE vector containing FRT::SA::51-bp Artificial exon::3xStop::SL2::SapI Insertion sites (to clone new driver with 3'UTR in slot 5 and SEC in slot 6)::reverse FRT3 to swap driver via RMCE

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-pleCopyCatcher

    Plasmid

    #174061

    Purpose

    CopyCatcher insertion donor for the pale locus

    Depositor

    Ethan Bier

    Article

    Li et al Nat Commun. 2021 May 11;12(1):2625. doi:

    Insert

    Pale (Pale Fly, Synthetic)
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-sadCas9-VP64

    Plasmid

    #115790

    Purpose

    An AAV vector expressing S. aureus dCas9 fused to VP64.

    Depositor

    Nadav Ahituv

    Article

    Matharu et al Science. 2018 Dec 13. pii: science.aau06

    Insert

    Sa-dCas9
    

    Use

    AAV

    Tags

    NLS and NLS-VP64

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVS1-Puro-CAG-mNG2(1-10)

    Plasmid

    #206042

    Purpose

    Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

    Insert

    AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
    

    Use

    CRISPR and Synthetic Biology; Donor template

    Expression

    Mammalian

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. OmpA-mNG-sfTq2ox

    Plasmid

    #124220

    Purpose

    Periplasmic OM bound mNeongreen superfolder mTurquoise2 ox tandem, ~40 % energy transfer. pNM092 - pTHV037-Ompa-SA1-177-(SA-1)-LEDPPAEL-mNG-sfTq2ox,

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    OmpA-mNG-sfTq2ox
    

    Expression

    Bacterial

    Available Since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT077

    Plasmid

    #137879

    Purpose

    Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT076

    Plasmid

    #137880

    Purpose

    Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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