We narrowed to 2,603 results for: ubiquitin

1. pLenti-myc-GLUT4-mCherry

   Plasmid

   #64049

   Purpose

   3rd generation lentiviral transfer plasmid. Reporter for GLUT4 translocation

   Depositor

   Weiping Han

   Article

   Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

   Insert

   GLUT4 (Slc2a4 Mouse)
   

   Use

   Lentiviral

   Tags

   c-Myc and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   July 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-28a-His6-SUMO-Munc18-1wt

   Plasmid

   #135550

   Purpose

   Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   Munc18-1wt
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ142-ZmUbi-tRNA

   Plasmid

   #158578

   Purpose

   Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 and pYPQ132-tRNA2.0; assembly of 2 gRNAs

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Maize ubiquitin 1

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET11a-SUMO1

   Plasmid

   #53138

   Purpose

   E.coli expression of untagged mature human SUMO1 (amino acids 1-97)

   Depositor

   Frauke Melchior

   Article

   Pichler et al Cell. 2002 Jan 11;108(1):109-20.

   Insert

   SUMO1 (SUMO1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mature form (amino acids 1-97)

   Promoter

   T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. phage ubc nls ha pcp gfp

   Plasmid

   #64539

   Purpose

   Used to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.

   Depositor

   Jeffrey Chao

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   PCP
   

   Use

   Lentiviral

   Tags

   EGFP, FactorXa site, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUbi-MAD7

   Plasmid

   #170133

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al J Genet Genomics. 2021 May 3. pii: S1673

   Insert

   MAD7(ErCas12a)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   maize Ubiquitin-1

   Available since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-HA-BAP1

   Plasmid

   #22539

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   BAP1 (BAP1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. A3A-PBE

   Plasmid

   #119768

   Purpose

   Conversion of C to T in wheat plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   APOBEC3A-nCas9-NLS-UGI-NLS
   

   Use

   Tags

   NLS (flanking UGI)

   Expression

   Plant

   Mutation

   nCas9 has a mutation D10A and E945K in wild type …

   Promoter

   maize Ubiquitin-1

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pH-A3A-PBE

   Plasmid

   #119774

   Purpose

   Conversion of C to T in rice plants transformation

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   APOBEC3A-nCas9-NLS-UGI-NLS
   

   Use

   Tags

   NLS (flanking UGI)

   Expression

   Plant

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   maize Ubiquitin-1

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRT498-OtUBD(V203D)

    Plasmid

    #190090

    Purpose

    Expresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

    Depositor

    Mark Hochstrasser

    Article

    Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

    Insert

    OtUBD(V203D)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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