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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6,927 results for: 11

Showing: 6421 - 6450 of 6927 results

  1. pTSlb-Q758C

    Plasmid
    #60729
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758C
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60729-plasmid-map-sequence-id-99392

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  13. pUAST-HA, Pbl-A

    Plasmid
    #69792
    Purpose
    Pebble isoform A in P element-based pUAST vector for Gal4-regulated expression in Drosophila.
    Depositor
    Arno Muller
    Article
    van Impel et al Development. 2009 Mar;136(5):813-22. doi
    Insert
    Pebble - Drosophila guanine nucleotide exchange factor
    Use
    P element-based puast vector for gal4-regulated e…
    Tags
    HA tag
    Expression
    Insect
    Mutation
    Encodes Pbl (NP_729306.1) lacking last 9 amino ac…
    Promoter
    hsp70 promoter
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pTSara-R756K

    Plasmid
    #60727
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756K. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    60727-plasmid-map-sequence-id-97715

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  21. pTSlb-R756K

    Plasmid
    #60731
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756S
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60731-plasmid-map-sequence-id-99358

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  22. pTSara-N748D

    Plasmid
    #60726
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation N748D. Contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    60726-plasmid-map-sequence-id-97714

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  24. AAV r3.45

    Plasmid
    #233684
    Purpose
    An evolved Adeno-associated viral variant, enhances gene delivery and gene targeting in neural stem cells
    Depositor
    David Schaffer
    Article
    Jang et al Mol Ther. 2011 Apr;19(4):667-75. doi: 10
    Insert
    Modified AAV2 Rep, Cap
    Use
    AAV
    Tags
    Expression
    Mutation
    insertion of LATQVGQKTA (where LA and A are linke…
    Promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    233684-plasmid-map-sequence-id-469929

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  28. L2096-GPA-BRET-Biosensor-Retrovirus-TD146

    Plasmid
    #222878
    Purpose
    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with BRET (split NanoLuciferase reconstitution [11S/114 fragment]). WT Glycophorin A (GPA) scaffold.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-NanoLuc114-CyOFP1-T2A-FKBP-GPA-NanoLuc11S
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    MSCV LTR
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222878-plasmid-map-sequence-id-446610

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  30. L2085-WT-GPA-NLuc-Biosensor-Retrovirus-TD135

    Plasmid
    #222875
    Purpose
    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). WT Glycophorin A (GPA) scaffold.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(WT)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    MSCV LTR
    Available since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    222875-plasmid-map-sequence-id-441838

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Showing: 6421 - 6450 of 6927 results