We narrowed to 8,459 results for: Ott

1. hAAVS1-gs4_EF1a-Cas9-2A-GFP

   Plasmid

   #118414

   Purpose

   CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

   Depositor

   Mark Denham

   Article

   Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

   Insert

   gRNA AAVS1 (AAVS1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV 3xFLAG-UBR5

   Plasmid

   #231238

   Purpose

   Flag-tagged wild-type UBR5 over-expression construct for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

   Insert

   UBR5 (UBR5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCasTet-λ

   Plasmid

   #128318

   Purpose

   Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

   Depositor

   Christopher Johnston

   Article

   Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

   Insert

   TetR and pTetR/TetO
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Constitutive expression of cas9 and anhydro-tetra…

   Promoter

   tet promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. OA-986F (AAEL003877-dCas9-VPR)

   Plasmid

   #183993

   Purpose

   Expresses dCas9-VPR under the Ae. aegypti polyubiquitin promoter

   Depositor

   Omar Akbari

   Article

   Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

   Insert

   AAEL003877-dCAS9-VPR
   

   Use

   CRISPR

   Tags

   dCas9-VPR

   Expression

   Insect

   Mutation

   Promoter

   AAEL003887

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP

   Plasmid

   #113888

   Purpose

   Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFP

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Avi-His6

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. VCP(A232E)-EGFP

   Plasmid

   #23973

   Depositor

   Nico Dantuma

   Article

   Tresse et al Autophagy. 2010 Feb 22. 6(2):.

   Insert

   Valosin containing protein (VCP Human)
   

   Use

   Tags

   6xHis, EGFP, and HA

   Expression

   Mammalian

   Mutation

   A232E

   Promoter

   Available since

   Feb. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV EFIa DIO LMO11 (GeNL_SS-ChRmine)

   Plasmid

   #219426

   Purpose

   Cre-dependent expression of luciferase opsin fusion protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

   Insert

   mNeonGreen, ISN-IE, ChRmine
   

   Use

   AAV

   Tags

   Gaussia luciferase signal sequence

   Expression

   Mutation

   Promoter

   EF-1alpha

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p6351 MSCV-CMV-CMV-Flag-HA-NSD3

   Plasmid

   #31357

   Depositor

   Peter Howley

   Article

   Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

   Insert

   NSD3 (NSD3 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNAintron-CDC50A-c3xFLAG

   Plasmid

   #215702

   Purpose

   Produces human CDC50A-c3xFLAG, a chaperone to phosphatidylserine flippase complexes

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CDC50A (TMEM30A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pQE32-pr-mEosFP (mEosFP-V69T)

    Plasmid

    #99213

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEosFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    monomeric variant with mutation V69T for primed c…

    Promoter

    T5

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. T-2A-EGFP-PGK-Puro

    Plasmid

    #83344

    Purpose

    Donor vector to knocin EGFP into human Brachyury(T) locus

    Depositor

    James Thomson

    Article

    Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

    Insert

    Human Brachyury knockin homology arms (TBXT Human)
    

    Use

    Tags

    2A-EGFP

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. SauKKHCas9 PE2*

    Plasmid

    #169852

    Purpose

    Mammalian Expression, sauCas9-KKH based prime editor

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SaCas9KKH-N580A-M-MLV
    

    Use

    Tags

    bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSIN4-EF2-N2L

    Plasmid

    #21163

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Nanog, Lin28 (NANOG, LIN28A Human)
    

    Use

    Lentiviral; Reprogramming

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAcGFP1-N1-RvCAHS3-GS

    Plasmid

    #192460

    Purpose

    Expression of RvCAHS3-GS_linker-AcGFP1 in mammalian cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS3
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAX-mScarlet-CIP4

    Plasmid

    #178364

    Purpose

    Expresses CIP4 c-terminal labeled with mScarlet in mammalian cells

    Depositor

    Erik Dent

    Article

    Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

    Insert

    Cdc42 Interacting Protein 4 (TRIP10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDule2-Acd82

    Plasmid

    #174100

    Purpose

    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

    Depositor

    Ryan Mehl

    Article

    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

    Insert

    Acridone 82 tRNA synthetase and tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N311S, C313G, V366A, W382T

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pF420

    Plasmid

    #123659

    Purpose

    Produces cofactor F420 in E. coli

    Depositor

    Colin Jackson

    Article

    Bashiri et al Nat Commun. 2019 Apr 5;10(1):1558. doi:

    Inserts

    CofD
    FbiB
    FbiD
    FbiC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_R0040

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSET-pr-mEos3.2 (mEosFP3.2-A69T)

    Plasmid

    #99224

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos3.2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A69T for primed conversion applications

    Promoter

    T7

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. GEF-H1 FLARE191.pa

    Plasmid

    #134931

    Purpose

    GEF-H1 biosensor that has GEF activity.

    Depositor

    Klaus Hahn

    Article

    Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

    Insert

    GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
    

    Use

    Tags

    None.

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV5 MEKK3 WT

    Plasmid

    #12186

    Depositor

    Gary Johnson

    Article

    Blank et al J Biol Chem. 1996 Mar 8. 271(10):5361-8.

    Insert

    MEKK3 (Map3k3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only