We narrowed to 9,006 results for: Pol;

1. pCellFree_G03 PDGFRB

   Plasmid

   #67130

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PDGFRB (PDGFRB Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pJin-290

   Plasmid

   #125718

   Purpose

   ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-C

   Depositor

   Bryan Dickinson

   Article

   Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

   Insert

   PCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMH-HT_SsCHAD

   Plasmid

   #127783

   Purpose

   Expresses SsCHAD in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSCALPS_miR-146aSponge_mCherry_GFP

   Plasmid

   #149718

   Purpose

   Lentiviral vector expressing a miR-146a decoy sponge

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   14X miR-146a binding sites
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   G to T mutation in position 75 of the insert

   Promoter

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPRIME-TET-GIN-recipient

   Plasmid

   #11656

   Purpose

   3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP-IRES-Neo expression

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP, IRES, and Neo

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. 2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

   Plasmid

   #172224

   Purpose

   Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX4T-1-human RNF146 RING-WWE (30-183)

   Plasmid

   #132611

   Purpose

   expresses human RNF146 RING-WWE (30-183) in E. coli

   Depositor

   Wenqing Xu

   Article

   DaRosa et al Nature. 2015 Jan 8;517(7533):223-6. doi:

   Insert

   RNF146 RING-WWE (30–183)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330(puro) Rosa26-H3F3B

   Plasmid

   #73131

   Purpose

   Expression in Mammalian Cells, Cas9 Nuclease to analize translocations

   Depositor

   Agnel Sfeir

   Article

   Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

   Insert

   spCas9
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-DiB2

   Plasmid

   #168801

   Purpose

   Fluorogen activating protein (FAP) DiB2

   Depositor

   Alexander Mishin

   Article

   Bozhanova et al Chem Sci. 2017 Oct 1;8(10):7138-7142. do

   Insert

   Fluorogen activating protein DiB2
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMX156

    Plasmid

    #29482

    Depositor

    Linda Wordeman

    Article

    Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

    Insert

    Kif2A-beta (Kif2a Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. sm-9xGCN4-Renilla-MS2

    Plasmid

    #178009

    Purpose

    Expresses Spaghetti Monster (sm) 9xGCN4-Renilla mRNA reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Gomez-Puerta et al Elife. 2022 Jun 22;11. pii: 75580. doi:

    Insert

    sm-9xGCN4
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET:GACG:GFP

    Plasmid

    #60735

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Psy074

    Plasmid

    #99504

    Purpose

    Bacterial expression of LIPS M1+TE

    Depositor

    Jay Keasling

    Article

    Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

    Insert

    LIPS M1+TE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCk1

    Plasmid

    #136695

    Purpose

    Acceptor plasmid for odd level position 1, lacZ blue-white screening. Part of the OpenPlant toolkit.

    Depositor

    Nicola Patron

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    lacZ
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLMB577

    Plasmid

    #83778

    Purpose

    Phenylalanine biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    promoter of RL1860
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. H2B-2A-GFP-IRESpuro2

    Plasmid

    #183746

    Purpose

    Expresses H2B-2A-GFP

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    H2B (H2BC16P Human)
    

    Use

    Tags

    GFP with 2A protease cleavage site between H2B an…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a - CPX

    Plasmid

    #89620

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Cytosolic Tryparedoxin peroxidase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. rat APOBEC1 E63A

    Plasmid

    #112861

    Purpose

    plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells

    Depositor

    Silvestro Conticello

    Article

    Chieca et al bioRxiv 2021

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Glutamic acid 63 to Alanine

    Promoter

    CMV promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330_SpCas9_USP7 Exon 3 gRNA

    Plasmid

    #131257

    Purpose

    pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    humanised S. pyogenes Cas9 nuclease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV5 MYC-Smurf2 (FF29/30A) C716A

    Plasmid

    #24605

    Depositor

    Jeff Wrana

    Article

    Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

    Insert

    Smurf2 (SMURF2 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    F29A, F30A, C716A

    Promoter

    Available since

    May 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits