We narrowed to 62,913 results for: cloning

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Ezh2[SET]-dCas9

Plasmid

#100087

Purpose

Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Publication

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

Ezh2[SET] (Ezh2 Mouse)

Use

CRISPR

Tags

3XFLag-NLS-Ezh2[SET]-dCas9-NLS

Expression

Mammalian

Mutation

Ezh2[SET] includes aa 482-746

Promoter

CMV

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pL2Cas9

Plasmid

#98841

Purpose

Genome engineering of virulent phages

Depositor

Sylvain Moineau

Publication

Lemay et al ACS Synth Biol. 2017 Mar 30. doi: 10.102

Insert

tracr/Cas9

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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POLD1_pLX307

Plasmid

#98358

Purpose

Lentiviral expression of POLD1

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

POLD1 (POLD1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTRANS_220

Plasmid

#91113

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pTRANS_220d

Plasmid

#91114

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMOD_A1110

Plasmid

#91031

Purpose

Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9

Use

CRISPR

Promoter

ZmUbi

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pUB-Cas9

Plasmid

#86556

Purpose

For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)

Depositor

Andreas Weber

Publication

Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

Insert

Cas9

Use

CRISPR

Expression

Plant

Mutation

Cas9 is codon optimized for C. reinhardtii

Promoter

UBIQUITIN10

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SETDB1_WT

Plasmid

#82291

Purpose

Gateway Donor vector containing SETDB1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SETDB1 (SETDB1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_NOTCH1_ICN

Plasmid

#82087

Purpose

Gateway Donor vector containing NOTCH1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NOTCH1 (NOTCH1 Human)

Use

Gateway Cloning

Mutation

ICN (AA1761-2556 present)

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBIG2ab

Plasmid

#80616

Purpose

pBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Publication

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pMA7-SacB

Plasmid

#79968

Purpose

TM-MAGE strain

Depositor

Morten Sommer

Publication

Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

Inserts

Lambda Red recombinase beta subunit
DNA adenine methylase
Levansucrase

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available since

Sept. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDEST-mCherry-LacR-PALB2

Plasmid

#71112

Purpose

mammalian expession of mCherry-LacR-PALB2

Depositor

Daniel Durocher

Publication

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV/TO

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-TAPP1-PH

Plasmid

#67662

Purpose

Mammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-TAPP1-PH

Expression

Mammalian

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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MSP1594

Plasmid

#65775

Purpose

Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

Depositor

Keith Joung

Publication

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS

Use

CRISPR

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1-V5-hTEV

Plasmid

#65800

Purpose

expresses human codon-optimised TEV protease

Depositor

Douglas Green

Publication

Oberst et al J Biol Chem. 2010 May 28;285(22):16632-4

Insert

V5-human TEV

Tags

V5

Expression

Mammalian

Mutation

codon-optimised to express in mammalian cells

Promoter

CMV

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5

Plasmid

#64613

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Ctnnb1 (Ctnnb1 Mouse)

Use

Lentiviral

Tags

V5

Mutation

S33A, S37A, T41A, S45A

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-ZMYND11

Plasmid

#65402

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

ZMYND11 (ZMYND11 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only
Luciferase-pcw107-V5

Plasmid

#64649

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Tags

V5

Promoter

PGK

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA-RanWT-mRFP1-polyA

Plasmid

#59750

Purpose

Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Publication

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran-mRFP1

Expression

Mammalian

Promoter

CMV and T7

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCCM935

Plasmid

#58202

Purpose

Expresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR experiments.

Depositor

Craig Mello

Publication

Kim et al Genetics. 2014 May 30. pii: genetics.114

Insert

unc-22 sgRNA

Expression

Worm

Promoter

U6

Available since

July 30, 2014

Availability

Academic Institutions and Nonprofits only