We narrowed to 5,622 results for: crispr cas9 grna plasmid

1. (326) pAAV SV40-ZsGreen U6-gRNA

   Plasmid

   #163020

   Purpose

   Fluorescent reporter for Sa gRNA (Bsa1 sites)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Zs Green
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI

   Plasmid

   #189923

   Purpose

   AAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   SaABE8e V106W
   

   Use

   AAV

   Tags

   Expression

   Mutation

   nSaCas9 KKH, TadA ABE8e V106W

   Promoter

   EFS

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-EFS-SaABE8eV106W-bGH-U6-sgRNA-BsmBI

   Plasmid

   #189924

   Purpose

   AAV genome encoding SaABE8eV106W and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   SaABE8e V106W
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SaCas9 D10A, TadA ABE8e V106W

   Promoter

   EFS

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. LLP773_pLenti-6xHRBKET-sgRNA

   Plasmid

   #211766

   Purpose

   Multiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   gRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   pCMV and U6

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiCRISPRv2-sgCD20

   Plasmid

   #209749

   Purpose

   Lentiviral transfer plasmid to express Cas9 and a gRNA targeting the human CD20 gene, MS4A1.

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Insert

   MS4A1 (MS4A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-sgRNA-Cre-GpNLuc

   Plasmid

   #208382

   Purpose

   Derived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Type

   Empty backbone

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-US-ECasE

   Plasmid

   #83961

   Purpose

   Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.

   Depositor

   Eleanor Chen

   Insert

   ERT2-spCas9-ERT2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   please see depositor comments below

   Promoter

   U6 (gRNA), EF1a (ERT2-Cas9-ERT2)

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

   Plasmid

   #154430

   Purpose

   hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

   Depositor

   David Liu

   Article

   Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

   Insert

   hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   VRQR point mutations in SpCas9

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-NQL005-SOX2-sgRNA

   Plasmid

   #175553

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse SOX2 locus.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   spCas9-nuclease and sgRNA against mouse SOX2 STOP Codon
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-NQL007-NANOG-sgRNA

    Plasmid

    #175555

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nuclease and sgRNA against mouse NANOG STOP Codon
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiCRISPRv2-scr

    Plasmid

    #169795

    Purpose

    Expresses Cas9 and guide RNA with untarget sequence generated by scramble of the target sequence of LentiCRISPRv2-ACTB-C1..

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    Scrambled guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiCRISPRv2-ACTB-C1

    Plasmid

    #169796

    Purpose

    Expresses Cas9 and guide RNA for ACTB gene in human

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    guide RNA for ACTB gene
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

    Plasmid

    #168290

    Purpose

    CRISPR-activator Suntag-P65-HSF1 (SPH)

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    dCas9, Suntag-P65-HSF1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-3xsgMyo7b-CMV-5’dCas9-SDS-BD10-SV40pA

    Plasmid

    #216322

    Purpose

    Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    Split Cas9-VPR + splice donor site + gRNAs targeting the murine Myo7b locus for transactivation
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNOC-ARS-CRISPR-v2

    Plasmid

    #99863

    Purpose

    Expresses Cas9-Nlux and sgRNA. Compact backbone contains CEN/ARS. Hygromycin selection marker.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis

    Tags

    Expression

    Mutation

    Promoter

    Ribi

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. UCK2_Deletion_gRNA_1

    Plasmid

    #204672

    Purpose

    Dual gRNA plasmid for UCK2 deletion

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    uridine-cytidine kinase 2 (UCK2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. UCK2_Deletion_gRNA_2

    Plasmid

    #204673

    Purpose

    Dual gRNA plasmid for UCK2 deletion

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    uridine-cytidine kinase 2 (UCK2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiCRISPRv2-KLF7-1

    Plasmid

    #169797

    Purpose

    Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene.

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    guide RNA for KLF7 neighbouring region
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNSA-ARS-CRISPR-BlastR

    Plasmid

    #101009

    Purpose

    Expresses Cas9-Nlux and sgRNA and contains CEN/ARS. CCMP537 LDSP promoter driven Blasticidin resistance gene.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMK

    Plasmid

    #62818

    Purpose

    Bacterial constitutive S. pyogenes Cas9 expression plasmid

    Depositor

    Sébastien Rodrigue

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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