We narrowed to 24,612 results for: Nes

1. pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant

   Plasmid

   #101213

   Purpose

   Bacterial expression plasmid for SpCas9 + K855A variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K855A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K855A

   Promoter

   T7

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. PatoG-OG241

   Plasmid

   #72839

   Purpose

   Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the native atoDAEB promoter (Pato) from E. coli DH5A. OG241 backbone.

   Depositor

   Chris Barnes

   Article

   Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

   Insert

   PatoG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PatoG

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. KAPCA_HUMAN_D0

   Plasmid

   #79741

   Purpose

   This plasmid encodes the kinase domain of KAPCA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Markus Seeliger, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   KAPCA
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. KIF5C(1-560)-EF(C)

   Plasmid

   #61675

   Purpose

   Non-fluorescent, truncated kinesin-1 fused to EF Hand linker

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   KIF5C(1-560)-EF(C) (Kif5c Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. KIF5C(1-560)-IA

   Plasmid

   #61671

   Purpose

   Non-fluorescent, truncated kinesin-1 fused to IA linker

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   KIF5C(1-560)-IA (Kif5c Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMDS1

   Plasmid

   #239460

   Purpose

   For simultaneous monitoring of both transcription and translation of genes in plant tissues: C-terminus fusion

   Depositor

   Miguel de Lucas

   Article

   Banda et al Sci. Adv., 11, eadw9153 (2025)

   Type

   Empty backbone

   Use

   Tags

   VENUS:3xHA:2A:mTurqN7

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available Since

   Oct. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMDS2

   Plasmid

   #239459

   Purpose

   Simultaneous monitoring of transcription and translation of genes in plant tissues: N- terminus fusion

   Depositor

   Miguel de Lucas

   Article

   Banda et al Sci. Adv., 11, eadw9153 (2025)

   Type

   Empty backbone

   Use

   Tags

   3xHA:VENUS:2A:mTurqN7

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available Since

   Aug. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CMV-MFSD6-Puro

   Plasmid

   #239953

   Purpose

   Lentiviral vector to generate MFSD6 stable expressing cell line under CMV promoter

   Depositor

   Jan Carette

   Article

   Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

   Insert

   MFSD6
   

   Use

   Lentiviral

   Tags

   myc-flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUSE-rIgG-Fc2-MFSD8(L9)-Zeo

   Plasmid

   #239947

   Purpose

   Expression of MFSD8 loop 9 tagged with rabbit IgG Fc2

   Depositor

   Jan Carette

   Article

   Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

   Insert

   Fc2-MFSD8(L9)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CMV-MFSD8-Puro

    Plasmid

    #239954

    Purpose

    Lentiviral vector to generate MFSD8 stable expressing cell line under CMV promoter

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    MFSD8
    

    Use

    Lentiviral

    Tags

    myc-flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDF_AaRaf1/Raf2/BSD2

    Plasmid

    #229515

    Purpose

    Expresses Anthoceros agrestis chaperones: Raf1,Raf2,BSD2

    Depositor

    Laura Gunn

    Article

    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

    Inserts

    Rubisco accumulation factor 1
    Rubisco accumulation factor 2
    BSD2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N terminus chloroplast transit peptide truncation

    Promoter

    T7

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. UBQ10pro-nlsABACUS2-400n-NosT

    Plasmid

    #203728

    Purpose

    Binary vector for the transformation of the nlsABACUS2-400n biosensor for (+)-Abscisic acid into Arabidopsis

    Depositor

    Alexander Jones

    Article

    Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

    Insert

    nlsABACUS2-400n
    

    Use

    Synthetic Biology

    Tags

    Myc

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10pro 1500bp

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEMS2371

    Plasmid

    #225637

    Purpose

    High Copy number plasmid containing Ple388 (PITX3 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Fornes et al Nucleic Acids Res. 2023 Jul 5;51(W1):W37

    Insert

    Ple388 (PITX3 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-U6-E690_guide-CBh-hSpCas9

    Plasmid

    #188545

    Purpose

    Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid E690

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    T2A-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-U6-W308_guide-CBh-hSpCas9

    Plasmid

    #188546

    Purpose

    Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid W308

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    T2A-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-U6-L101_guide-CBh-hSpCas9

    Plasmid

    #188547

    Purpose

    Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid L101

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    T2A-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-myc-GFP-TNRC6A-SD-WT

    Plasmid

    #215897

    Purpose

    Expression of TNRC6A-silencing domain (SD)

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1709

    Promoter

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-WT

    Plasmid

    #215898

    Purpose

    Expression of TNRC6A-CIM1 domain

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1528

    Promoter

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-myc-GFP-TNRC6A-SD-CIM2-WT

    Plasmid

    #215899

    Purpose

    Expression of TNRC6A-CIM2 domain

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1529-1709

    Promoter

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1332A

    Plasmid

    #215900

    Purpose

    Expression of TNRC6A-CIM1 domain with S1332A mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1528, S1332A

    Promoter

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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