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pQTEV-MGC20398

Plasmid

#34825

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

MGC20398 (ZNF830 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBac(3xP3-gTc’v; pUb:lox-mYFP-lox-H2BmCherry)

Plasmid

#119064

Purpose

Cre/lox flip-out construct for marking cell clones in Tribolium castaneum

Depositor

Michalis Averof

Article

Gilles et al Development. 2019 Jan 4;146(1). pii: dev

Inserts

pBacL
pUb regulatory sequence
loxN
mYFP
loxN
H2B-mcherry
SV40polyA
Tc-vermilion
3xP3 TATA
pBacR

Expression

Insect

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM4Z globin NB Probe

Plasmid

#120390

Purpose

Allows in vitro SP6 Polymerase driven transcription of a beta globin northern blot probe

Depositor

Niels Gehring

Article

Voigt et al Nat Protoc. 2019 May;14(5):1603-1633. do

Insert

beta globin partial 3' UTR

Use

Unspecified

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-125b-3p-Decoy.hPGK.GFP.WPRE

Plasmid

#46582

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-125b-3p Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pLN426 (FuGW-G5p-mKate2)

Plasmid

#105183

Purpose

Module 3 - GAL4 synthetic promoter (with five binding sites) drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G5p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-128-Decoy.hPGK.GFP.WPRE

Plasmid

#46584

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-128 Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
RASL10A

Plasmid

#55674

Purpose

expression of mouse RASL10A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RASL10A (Rasl10a Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pEF-DEST51 LacZ

Plasmid

#123292

Purpose

Mammalian expression of control truncated LacZ

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

LacZ

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-RS12

Plasmid

#52650

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS12

Expression

Mammalian

Mutation

deleted R40/R41/K42 from GCaMP3

Promoter

T7

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.GFP.miR-152-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85872

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-152-3p Sensor (MIR152 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac-LacI-LAP

Plasmid

#114034

Purpose

Production of baculovirus with LacI-LAP

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

LacI-LAP

Expression

Insect

Mutation

none

Promoter

CMV

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pETM6-Pc4CL-PhCHS-CmCHI

Plasmid

#73397

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-zEPLIN

Plasmid

#40957

Depositor

Elizabeth Luna, Scott Maul

Article

Maul et al Gene. 2001 Jan 10;262(1-2):155-60.

Insert

EPLIN (lima1 Zebrafish)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pLN553 (FuGW-S(MAFK)p-Sponge7)

Plasmid

#105203

Purpose

Module 2 - synthetic promoter MAFK drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(MAFK)p-Sponge7

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMCh-1468

Plasmid

#82418

Purpose

Plasmid for expression of HA-tagged Nematostella vectensis GW182 in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvGW182

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-EF09

Plasmid

#52640

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF09

Expression

Mammalian

Mutation

D366N mutation from GCaMP3 sequence

Promoter

T7

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
Human NCKX3 (pcDNA3.1+)

Plasmid

#75205

Purpose

Mammalian expression of SLC24A3

Depositor

Jonathan Lytton

Article

Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

Insert

SLC24A3 (SLC24A3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-16-2-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85876

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-16-2-3p Sensor (MIR16-2 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCVD447

Plasmid

#78569

Purpose

E. coli E2348/69 LEE region (Type 3 Secretion System) cloned into pBluescriptKS.

Depositor

James Kaper

Article

Jarvis et al Proc Natl Acad Sci U S A. 1995 Aug 15;92

Insert

lee operon

Use

Cloning vector

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pQTEV-NEUGRIN

Plasmid

#34804

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

NEUGRIN (NGRN Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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