Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 15,864 results for: cas9

Clear keywords
Showing: 4301 - 4320 of 15864 results

  5. SpCas9-NG-miniCGBE1

    Plasmid
    #198538
    Purpose
    Expresses human codon-optimized SpCas9-NG-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-miniCGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-miniCGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198538-plasmid-map-sequence-id-388888

    Enlarge

  6. SpCas9-NRTH

    Plasmid
    #198541
    Purpose
    Expresses human codon-optimized SpCas9-NRTH and blasticidin resistance: EFS promoter-SpCas9-NRTH-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRTH
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198541-plasmid-map-sequence-id-388886

    Enlarge

  7. SpCas9-NRCH

    Plasmid
    #198542
    Purpose
    Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198542-plasmid-map-sequence-id-388906

    Enlarge

  9. SpCas9 dFE

    Plasmid
    #190137
    Purpose
    Expresses SpCas9-T4pol (dFE)
    Depositor
    Timothy Lu
    Publication
    Nakade et al bioRxiv 2022.12.05.518807
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-T4pol (dFE)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Codon Optimized for mammalian cells
    Promoter
    CBh
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190137-plasmid-map-sequence-id-382840

    Enlarge

  11. pCAG-T3-HypaCAS9-pA

    Plasmid
    #131467
    Purpose
    Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Publication
    Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon optimized HypaCas9
    Use
    CRISPR
    Tags
    Flag, NLS, and polyA
    Expression
    Mammalian
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131467-plasmid-map-sequence-id-262453

    Enlarge

  12. pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

    Plasmid
    #197504
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV-T7-ABE8e-NRCH-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197504-plasmid-map-sequence-id-386610

    Enlarge

  15. D10A-Cas9

    Plasmid
    #239383
    Purpose
    For circular RNA-mediated inverse prime editor using nCas9-D10A in HEK294T cells
    Depositor
    Caixia Gao
    Publication
    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:
    Insert
    nCas9-D10A
    Use
    CRISPR
    Tags
    BPNLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A in Cas9
    Promoter
    CMV
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239383-plasmid-map-sequence-id-481779

    Enlarge

  20. PB-TAC-Cas9

    Plasmid
    #120352
    Purpose
    piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Publication
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    tet-on and CBh
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120352-plasmid-map-sequence-id-237238

    Enlarge

Showing: 4301 - 4320 of 15864 results