We narrowed to 34,848 results for: CaS;

1. pACYC-Cas12c2-noncoding

   Plasmid

   #120877

   Purpose

   Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Noncoding sequences for CRISPR-Cas12c2
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

   Plasmid

   #107727

   Purpose

   Knock out of EB1 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221 Cas9-RFC5

   Plasmid

   #183200

   Purpose

   Entry clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   Cas9-RFC5
   

   Use

   CRISPR; Gateway entry clone

   Tags

   Expression

   Mutation

   n/a

   Promoter

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC92-pmax-dCas9VP96

   Plasmid

   #48224

   Purpose

   dCas9VP96 on pmax expression vector

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP96 activation domain
   

   Use

   CRISPR

   Tags

   HA Tag and VP96

   Expression

   Mammalian

   Mutation

   D10A;H840A

   Promoter

   CAGGS

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAT15546_nABE8e-SuperFi-Cas9

   Plasmid

   #184376

   Purpose

   Mammalian expression of SuperFi-Cas9 ABE8e base editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   nABE8e-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

   Promoter

   EF-1α core promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-LmoCas6-p300

   Plasmid

   #126486

   Purpose

   Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas6-p300
   

   Use

   CRISPR

   Tags

   Flag and Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEJS1026-pMCSG7-HpaCas9

   Plasmid

   #121540

   Purpose

   Expresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   HpaCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. ciCas9(L22)_pcDNA5

   Plasmid

   #100551

   Purpose

   Expresses ciCas9(L22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

   Insert

   ciCas9(L22)
   

   Use

   CRISPR; Frt, flp-in

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-TAC-OKMS-Cas9

   Plasmid

   #120353

   Purpose

   piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OKMS cassette
   

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Klf4 [10-483]

   Promoter

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS1027-pMCSG7-SmuCas9

    Plasmid

    #121541

    Purpose

    Expresses a 6X-His tagged type II-C Cas9 from S. muelleri in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    SmuCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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