We narrowed to 8,159 results for: Dos;

1. pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)

   Plasmid

   #101139

   Purpose

   Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZT-C13-R1

   Plasmid

   #62197

   Purpose

   Chr.13/CLYBL TALEN expression vector

   Depositor

   Jizhong Zou

   Article

   Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

   Insert

   Right CLYBL TALEN
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZT-C13-L1

   Plasmid

   #62196

   Purpose

   Chr.13/CLYBL TALEN expression vector

   Depositor

   Jizhong Zou

   Article

   Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

   Insert

   Left CLYBL TALEN
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 TAP DHX36

   Plasmid

   #53547

   Purpose

   mammalian expression of DHX36

   Depositor

   Kathleen Collins

   Article

   Sexton et al Mol Cell Biol. 2011 Feb;31(4):736-43. do

   Insert

   DHX36 (DHX36 Human)
   

   Use

   Tags

   TAP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV/D377Y-mPCSK9

   Plasmid

   #58376

   Purpose

   Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

   Depositor

   Jacob Bentzon

   Article

   Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

   Has Service

   AAV8

   Insert

   proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   D377Y

   Promoter

   HCRApoE/hAAT

   Available since

   Aug. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPPED

   Plasmid

   #162468

   Purpose

   Destination Expression vector for dicot plant prime editing

   Depositor

   Adam Bogdanove

   Article

   Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

   Inserts

   Cas9H840A
   attR gateway destination cassette
   

   Use

   Tags

   MLV reverse transcriptase

   Expression

   Plant

   Mutation

   Change histidine 840 to Alanine based sequence o…

   Promoter

   2x35S

   Available since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pM162

   Plasmid

   #173221

   Purpose

   Expresses ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP(1-9)-GB1 and Puro-T2A-MCP-mCherry-GFP11-GB1

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Inserts

   ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP (1-10)-GB1
   MCP-mCherry-GFP11-GB1
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-NLS-Cas9-6xHis

   Plasmid

   #62934

   Purpose

   Expression of NLS-Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   NLS-Cas9-6xHis
   

   Use

   Tags

   6xHis tag and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCVFLAG-ZFP423

   Plasmid

   #24764

   Depositor

   Bruce Spiegelman

   Article

   Gupta et al Nature. 2010 Mar 25;464(7288):619-23. do

   Insert

   Zinc Finger Protein 423 (Zfp423 Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pCMV-Frankenbody-FLAG-mEGFP

    Plasmid

    #175293

    Purpose

    Track mature and nascent FLAG tagged proteins in live cells

    Depositor

    Tim Stasevich

    Article

    Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

    Insert

    anti-FLAG frankenbody fused with mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-Cas9-6xHis

    Plasmid

    #62374

    Purpose

    Expression of Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9_6xHis
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiGuide_DKO

    Plasmid

    #183193

    Purpose

    Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

    Depositor

    Lang Li

    Article

    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, mU6

    Available since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Frankenbody-FLAG-mRuby2

    Plasmid

    #175294

    Purpose

    Track mature and nascent FLAG tagged proteins in live cells

    Depositor

    Tim Stasevich

    Article

    Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

    Insert

    anti-FLAG frankenbody fused with mRuby2
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pLJC6-2XFLAG-TMEM192

    Plasmid

    #104435

    Purpose

    Expresses 2XFLAG-TMEM192 in mammalian cells

    Depositor

    David Sabatini

    Article

    Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

    Insert

    TMEM-192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    2XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry

    Plasmid

    #218860

    Purpose

    10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hGCG-ICAM(1235)-nrxn1b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLJC6-3XHA-TMEM192

    Plasmid

    #104434

    Purpose

    Expresses 3XHA-TMEM192 in mammalian cells

    Depositor

    David Sabatini

    Article

    Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-Frankenbody-FLAG-SNAP

    Plasmid

    #175295

    Purpose

    Track mature FLAG tagged proteins in live cells

    Depositor

    Tim Stasevich

    Article

    Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

    Insert

    anti-FLAG frankenbody fused with SNAP-tag
    

    Use

    Tags

    SNAP-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pMOS003-lenti-CMV-GCaMPer

    Plasmid

    #65227

    Purpose

    ER calcium sensor for lentivirus production

    Depositor

    Brandon Harvey, Adam Cohen

    Article

    Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

    Insert

    GCaMPer
    

    Use

    Lentiviral

    Tags

    circularly permuted GFP

    Expression

    Mammalian

    Mutation

    GCaMP3 (D324G, D360G, D397G, D435G)

    Promoter

    CMV

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-BAF

    Plasmid

    #101772

    Purpose

    Expresses EGFP tagged BAF in human cells

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA-FLAG-METTL3-APPA

    Plasmid

    #160251

    Purpose

    Express catalytic inactive FLAG-METTL3 in human cells

    Depositor

    Alessandro Fatica

    Article

    Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changes in aa 395–398, DPPW → APPA

    Promoter

    CMV

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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