We narrowed to 5,529 results for: crispr cas9 grna plasmid

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AIO-GFP

Plasmid

#74119

Purpose

All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

Depositor

Steve Jackson

Article

Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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AIO-mCherry

Plasmid

#74120

Purpose

All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

Depositor

Steve Jackson

Article

Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pYZ173

Plasmid

#98410

Purpose

Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.lys9 (SPBC3B8.03 Fission Yeast)

Use

CRISPR

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ146

Plasmid

#98406

Purpose

Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.leu1 (leu1 Fission Yeast)

Use

CRISPR

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ164

Plasmid

#98408

Purpose

Cas9-gRNA his3 plasmid for his3 deletion in S. pombe

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.his3 (his3 Fission Yeast)

Use

CRISPR

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-HA-TadA8e-Sauri N aa1-438-inteinN-U6-Camk2d sgRNA7

Plasmid

#226678

Purpose

Expresses TadA8e and Sauri cas9N by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauri Cas9N, inteinN

Use

AAV

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDIV069

Plasmid

#247966

Purpose

CRISPR-Cas9 vector containing a 20 nt protospacer sequence to target the IS1 locus where the DIVERSIFY gene landing platform is integrated in Aspergillus nidulans. pyrG marker

Depositor

Uffe Mortensen

Insert

Cas9-AMA1-IS1-gRNA-pyrG-ori-AmpR

Use

CRISPR

Mutation

none

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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pJB172

Plasmid

#86992

Purpose

CRISPR/Cas9 in fission yeast using fluoride selection and targetting pil1

Depositor

Julien Berro

Article

Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

Insert

gRNA targeting pil1

Expression

Yeast

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF219_1

Plasmid

#72368

Purpose

Encodes gRNA for 3' target of human ZNF219 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Insert

ZNF219 (ZNF219 Human)

Use

CRISPR

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF219_2

Plasmid

#72369

Purpose

Encodes gRNA for 3' target of human ZNF219 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Insert

ZNF219 (ZNF219 Human)

Use

CRISPR

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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PX458_TGIF2_1

Plasmid

#72374

Purpose

Encodes gRNA for 3' target of human TGIF2 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Insert

TGIF2 (TGIF2 Human)

Use

CRISPR

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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PX458_TGIF2_2

Plasmid

#72375

Purpose

Encodes gRNA for 3' target of human TGIF2 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Insert

TGIF2 (TGIF2 Human)

Use

CRISPR

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF146_1

Plasmid

#72360

Purpose

Encodes gRNA for 3' target of human ZNF146 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Insert

ZNF146 (ZNF146 Human)

Use

CRISPR

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF146_2

Plasmid

#72361

Purpose

Encodes gRNA for 3' target of human ZNF146 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Insert

ZNF146 (ZNF146 Human)

Use

CRISPR

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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PX458_SSRP1_1

Plasmid

#72371

Purpose

Encodes gRNA for 3' target of human SSRP1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Insert

SSRP1 (SSRP1 Human)

Use

CRISPR

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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PX458_SSRP1_2

Plasmid

#72372

Purpose

Encodes gRNA for 3' target of human SSRP1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Insert

SSRP1 (SSRP1 Human)

Use

CRISPR

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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PX458_PBX2_1

Plasmid

#72357

Purpose

Encodes gRNA for 3' target of human PBX2 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Insert

PBX2 (PBX2 Human)

Use

CRISPR

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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PX458_PBX2_2

Plasmid

#72358

Purpose

Encodes gRNA for 3' target of human PBX2 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Insert

PBX2 (PBX2 Human)

Use

CRISPR

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-RNF2-ngRNA+41_EF1a-puroR

Plasmid

#207358

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

RNF2 +41 ngRNA

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCJ1023

Plasmid

#228763

Purpose

Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.

Depositor

Bradley Yoder

Insert

Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

human U6

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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