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pPB-hCMV1-T-V5-GR-2A-H2BmCherry-pA

Plasmid

#171501

Purpose

Overexpression of T(Brachyury)-GR

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

T

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-hCMV1-T-V5-GR-IRES-H2BmCherry-pA

Plasmid

#171502

Purpose

Overexpression of T(Brachyury)-GR

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

T

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NODAL-matDYK

Plasmid

#115258

Purpose

For mammalian expression of the human NODAL open reading frame with an internal DYK tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODAL-T7TS

Plasmid

#115250

Purpose

For in vitro transcription of human NODAL open reading frame RNA

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMA3998rc

Plasmid

#184851

Purpose

A retroviral vector with blasticidin resistance for cloning inserts between loxP sites

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Type

Empty backbone

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Rp51a Hyperstabilized 5'SS

Plasmid

#100988

Purpose

pUC18 Hyperstabilized Rp51a 5'SS between HindIII and EcoRI; ATG/GTATGT -> AAG/GTAAGT

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

5'ss mut ATG/GTATGT -> AAG/GTAAGT

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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RP51A-NoBS-NoPseudoBS

Plasmid

#100986

Purpose

RP51A w/ T7 promoter in HindIII/EcoRI sites of pUC18. Has WT 5'ss but mutant BS and no pseudo BS

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

BS mutated to GTTAGTG, pseudo BS mutated to GTTTG…

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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A0A0K2XRH9

Plasmid

#163240

Purpose

Inducible expression of UniProt identifier A0A0K2XRH9

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0K2XRH9

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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A0A0J6V898

Plasmid

#163185

Purpose

Inducible expression of UniProt identifier A0A0J6V898

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A0J6V898

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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K0NHF1

Plasmid

#163203

Purpose

Inducible expression of UniProt identifier K0NHF1

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

K0NHF1

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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I9UMF5

Plasmid

#163252

Purpose

Inducible expression of UniProt identifier I9UMF5

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

I9UMF5

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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F4G5A4

Plasmid

#163219

Purpose

Inducible expression of UniProt identifier F4G5A4

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

F4G5A4

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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H5VAZ0

Plasmid

#163280

Purpose

Inducible expression of UniProt identifier H5VAZ0

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

H5VAZ0

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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U6-U57A

Plasmid

#169927

Purpose

WT U6 with a U57A mutation

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

U6 U57A

Expression

Bacterial and Yeast

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pJT9RFR

Plasmid

#165067

Purpose

Introducing GAL promoters-controlled sequiterpene nerolidol-producing module and Y-FAST and in S. cerevisiae

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

T-RPL3>ERG20>P-GAL1-P-GAL2>Y-FAST>ErB1.2A-AcNES1>T-RPL41B

Expression

Yeast

Mutation

WT

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pISKP1-TIR1

Plasmid

#165061

Purpose

Integrative expression of SKP1-fusing rice auxin receptor gene OsTIR1

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1- T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFP105

Plasmid

#165060

Purpose

Integrative expression of nucleus-targeting yEGFP-AID*(mini auxin inducible degron)-CUP1

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1- T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFP104

Plasmid

#165059

Purpose

Integrative expression of yEGFP-AID*(mini auxin inducible degron)-CUP1

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJAID9L104

Plasmid

#165054

Purpose

Integrative expression of yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b_STS:P4S

Plasmid

#139797

Purpose

expression of biosynthetic enzyme STS in fusion with CC segment P4S

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Insert

STS:P4S

Expression

Bacterial

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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