164,577 results

1. pGWB540

   Plasmid

   #74874

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   No Promoter

   Available Since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ePPE-spG

   Plasmid

   #183096

   Purpose

   For plant prime editing in rice plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

   Insert

   Nls-nspGCas9-NC-nls-MLV-Nls
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A in SpGCas9

   Promoter

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mEmerald-Desmin-C-18

   Plasmid

   #54059

   Purpose

   Localization: Intermediate Filaments, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Desmin (DES Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. mNeonGreen-Giantin

   Plasmid

   #98880

   Purpose

   In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   Giantin
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHAGE2 Lnp-mCherry

   Plasmid

   #86687

   Purpose

   Lentivirus to stably express fluorescent human protein in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   LNPK (LNPK Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-TripleColour-StAR

   Plasmid

   #222695

   Purpose

   Competition assay

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP, miRFP703, dTomato

   Expression

   Mammalian

   Mutation

   PGK, TagBFP, dTomato without BsmBI cutsite

   Promoter

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTRE/VEGF/IRES/EGFP

   Plasmid

   #206211

   Purpose

   An expression vector of VEGF and EGFP under control of TRE promoter

   Depositor

   Masamichi Kamihira

   Article

   Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

   Insert

   vascular endothelial growth factor (Vegfa Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mTagBFP2-PCNA-19-SV40NLS-4

   Plasmid

   #55320

   Purpose

   Localization: Nucleus, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Use

   Tags

   3xSV40 NLS and mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. Brm WT-Flag pShuttle

   Plasmid

   #122105

   Purpose

   Expression of full length Brm in mammalian cells

   Depositor

   Paul Herring

   Article

   Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

   Insert

   BRM (SMARCA2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMpGE013

    Plasmid

    #108681

    Purpose

    All-in-one genome editing vector in M. polymorpha (hygromycin resistant)

    Depositor

    Takayuki Kohchi, Shigeo Sugano

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1_Linear Tau 0N4R 3X Flag tag

    Plasmid

    #194171

    Purpose

    Insert contains linear tau 0N4R with a 3X Flag tag. Expresses linear protein tau 0N4R with a 3X Flag tag for Immunoprecipitation.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    Microtubule-Associated Protein Tau 0N4R
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CX3CR1-EGFP

    Plasmid

    #24061

    Depositor

    Dan Littman

    Insert

    CX3CR1 (Cx3cr1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDL278_P23-DsRed-Express2

    Plasmid

    #121505

    Purpose

    E. coli-streptococcal shuttle vector that expresses rfp

    Depositor

    Robert Shields

    Article

    Shields et al Appl Environ Microbiol. 2019 May 17. pii

    Insert

    DsRed-Express2
    

    Use

    E. coli streptococcal shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    P23

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SIRT3L-12

    Plasmid

    #13736

    Depositor

    John Denu

    Article

    Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

    Insert

    SIRT3 (SIRT3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a MISTIC

    Plasmid

    #85999

    Purpose

    bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    N/A
    

    Use

    Tags

    HIS6 and MISTIC

    Expression

    Bacterial

    Mutation

    codon optimzed for bacterial expression

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

    Plasmid

    #214995

    Purpose

    Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT2/LTR7-GFP

    Plasmid

    #62541

    Purpose

    LTR7-driving reporter

    Depositor

    Zsuzsanna Izsvak

    Article

    Wang et al Nature. 2014 Dec 18;516(7531):405-9. doi

    Insert

    LTR7-GFP
    

    Use

    Sleeping beauty transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR7

    Available Since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHR CD19 tandem CAR

    Plasmid

    #113019

    Purpose

    for lentivirus production of CD19 tandem CAR

    Depositor

    Ron Vale

    Article

    Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

    Insert

    CD19 CAR-P tandem FcR gamma intracellular and PI3k recruiting domain from CD19 (CD19 Synthetic, Mouse, Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

    Plasmid

    #112021

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO beta/alpha into TRAC HDRT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. human beta-catenin pcDNA3

    Plasmid

    #16828

    Purpose

    Mammalian expression of human beta-catenin with Flag tag

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge