We narrowed to 14,011 results for: flag

1. pUC57-ITR2-H1-sgCAG-SlugD10A-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #222858

   Purpose

   This plasmid codes for the Slug-KH nickase with a sg(CAG)6

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   Slug-KHD10A+sgCAG
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, ITR2 and ITR2

   Expression

   Mammalian

   Mutation

   KH variant and D10A mutation

   Promoter

   H1 and eCMV

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-ITR2-H1-sgGCA-SlugD10A-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #222860

   Purpose

   This plasmid codes for the Slug-KH nickase with a sg(GCA)6

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   Slug-KHD10A+sgGCA
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, ITR2 and ITR2

   Expression

   Mammalian

   Mutation

   KH variant and D10A mutation

   Promoter

   H1 and eCMV

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_Myc-HalM-P2A-FLAG-YFast-TEV-His-HalB

   Plasmid

   #225064

   Purpose

   Expression of HalM-modified HalA2 in human cells

   Depositor

   Wilfred van der Donk

   Article

   Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

   Insert

   HalA2
   

   Use

   Tags

   Multiple tags due to polycistronic expression

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG

   Plasmid

   #217321

   Purpose

   Purification of autocleavage-deficient Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells

   Depositor

   Yifan Wang

   Insert

   NLRP1
   

   Use

   Tags

   FLAG and His-TEV

   Expression

   Insect

   Mutation

   S969A

   Promoter

   polyhedrin

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXS-IRES-BLAST flag-CAD T456A S1406A S1859A

   Plasmid

   #169826

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported S6K, PKA, and Erk1/2 phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   T456A S1406A S1859A; TCCC -> AGTC silent mutat…

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXS-IRES-BLAST flag-CAD delta Loop1 and Loop2

   Plasmid

   #169833

   Purpose

   Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 and F1366 - P1376 in allosteric domain

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Deletions of amino acids R1398 - S1407 and F1366 …

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXS-IRES-BLAST flag-CAD delta Allosteric Domain

   Plasmid

   #169835

   Purpose

   Expresses C-terminal flag-tagged CAD with substitution of allosteric domain F1308 - C1455 with a (GGGS)X3 linker

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Substitution of amino acids F1308 - C1455 with a …

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔLD + ΔSS

   Plasmid

   #194610

   Purpose

   Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Linker + Small Subunit Domain

   Depositor

   Hannah Rabinowich

   Article

   Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

   Insert

   N-terminal 3X Flag Mouse C325A Caspase-9 Minus Sequence Encoding Ala 354 to Ser 454 (Casp9 Mouse)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Changed Cysteine 325 to Alanine to eliminate prot…

   Promoter

   CMV

   Available Since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXS-IRES-BLAST flag-CAD R1398A R1403A R1404A

   Plasmid

   #188134

   Purpose

   Expresses C-terminal flag-tagged human CAD R1398A R1403A R1404A in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXS-IRES-BLAST flag-CAD E1367A E1368A E1373A

    Plasmid

    #188144

    Purpose

    Expresses C-terminal flag-tagged human CAD E1367A E1368A E1373A in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV10-3xFLAG-prs-hMIGA2(170-593)-25GS-6xhis

    Plasmid

    #192835

    Purpose

    Express codon-optimized human MIGA2 fragment 170-593 with a GS-linker and 6xhis tag at C-terminus (lipid transfer construct) in Expi293 cells

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Use

    Tags

    3xFLAG-PreScission cleavage site and 6xhis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. psiCheck2-SV40p-5'UTR-mtIF3(SR)-3xFLAG-3'UTR-HSVTKp-mCherry

    Plasmid

    #182379

    Purpose

    Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Inserts

    mtIF3 (Mtif3 Mouse)
    mCherry
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Changed CDS sequence from 'ccacgttcaagtcacga…

    Promoter

    HSV TK promoter and SV40 promoter

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-CMV-Flag-PolB(K206A/K244A/T304I)-Puro

    Plasmid

    #177145

    Purpose

    Lentiviral vector expressing Flag-PolB(K206A/K244A/T304I) and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG E1322R

    Plasmid

    #166814

    Purpose

    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E1322R

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R K1277E

    Plasmid

    #166811

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association, K1277E abolishes UPA-UPA contacts

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    LL1193EE, P1214R, K1277E

    Promoter

    CMV

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG H1276G

    Plasmid

    #166812

    Purpose

    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H1276G

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG K1277E

    Plasmid

    #166813

    Purpose

    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K1277E

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-Flag-Polß(L301R/V303R/V306R)-IRES-puro

    Plasmid

    #128657

    Purpose

    mammalian expression of Flag-Polß(L301R/V303R/V306R) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L301R/V303R/V306R

    Promoter

    CMV

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJM591 EF1α 3xFLAG-NLS-VP64-ZF1-intN in TUPV2

    Plasmid

    #161567

    Purpose

    Constitutive expression of 3xFLAG-NLS-VP64-ZF1-intN under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-VP64-ZF1-intN
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJM505 EF1α 3xFLAG-NLS-DsRed-Express2-ZF1 in TUPV2

    Plasmid

    #161535

    Purpose

    Constitutive expression of 3xFLAG-NLS-DsRed-Express2-ZF1 under the EF1α promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-DsRed-Express2-ZF1
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJM553 ZF10x6-C 3xFLAG-NLS-DsDed-ZF1-PEST in TUPV2

    Plasmid

    #161540

    Purpose

    Inducible expression of 3xFLAG-NLS-DsDed-ZF1-PEST under the ZF10x6-C promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-DsRed-ZF1-PEST
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    ZF10x6-C

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA-GFP-AMA1-Flag(K546M/I547P/I548Y/I549Y)

    Plasmid

    #155308

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged AMA1 K546M/I547P/I548Y/I549Y variant

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfAMA1
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    K546M/I547P/I548Y/I549Y

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA-GFP-EBA175-Flag(M1423R/P1424V/Y1425W/Y1426H)

    Plasmid

    #155313

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V/Y1425W/Y1426H variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    M1423R/P1424V/Y1425W/Y1426H; insert codes for EBA…

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA-GFP-EBA175-Flag(E1418K/A1419R/P1424Q/Y1426S)

    Plasmid

    #155009

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    E1418K/A1419R/P1424Q/Y1426S; insert codes for EBA…

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRDAX-017 FLAG Venus173/155[1] IRES Venus173/155[2]

    Plasmid

    #130127

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pX330-Flag-SpCas9-HF1 (without sgRNA; with silent mutations)

    Plasmid

    #126755

    Purpose

    Expression plasmid for human codon-optimized increased fidelity SpCas9-HF1 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-CMV-SMCHD1-Exon1-11E.12D-14.47M-48-3xFLAG

    Plasmid

    #130684

    Purpose

    Expresses human SMCHD1 Exon1-11E.12D-14.47M-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-11E.12D-14.47M-48 (SMCHD1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D453_F489del, E653_G1960del

    Promoter

    CMV

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti-CMV-SMCHD1-Exon1-10A.11D-14.47M-48-3xFLAG

    Plasmid

    #130683

    Purpose

    Expresses human SMCHD1 Exon1-10A.11D-14.47M-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-10A.11D-14.47M-48 (SMCHD1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D409_E452del, E653_G1960del

    Promoter

    CMV

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLU-CMV-Flag-hSesn2 L373A/N376A/D385A/S387A

    Plasmid

    #111789

    Purpose

    Mammalian expression or 3rd generation lentivirus generation of hSesn2

    Depositor

    Jun Hee Lee

    Article

    Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

    Insert

    hSesn2 (SESN2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L373A/N376A/D385A/S387A

    Promoter

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1 E2F1 Flag(Δ193-359) (HindIII- BamHI- EcoRI)

    Plasmid

    #70667

    Purpose

    Human mutant of E2F1 lacking amino acids 193-359

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F1 lacking residues 193 to 359 (E2F1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    lacks amino acids 193-359

    Promoter

    cmv

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge