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PAP-delta10-cHIS

Plasmid

#21716

Depositor

Andrew Bohm

Article

Balbo et al Biochemistry. 2005 May 31. 44(21):7777-8

Insert

polyA polymerase (PAP1 Budding Yeast)

Tags

HIS

Expression

Bacterial

Mutation

30 amino acids off the c terminus

Available Since

Aug. 22, 2009

Availability

Academic Institutions and Nonprofits only
pMLS328

Plasmid

#73717

Purpose

C. elegans germline CRE expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

2xNLS-CRE

Use

Cre/Lox

Expression

Worm

Promoter

Peft-3

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pDR-GW AT1.03YEMK

Plasmid

#28004

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

AT1.03YEMK

Tags

mVenus and mseCFP

Expression

Yeast

Mutation

M60Y, G131E, K132M, 133K

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pDiGi

Plasmid

#59323

Purpose

Inducible expression of dsRed and GFP in bacteria

Depositor

Sophie Helaine, David Holden

Article

Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

Inserts

EGFP
dsRed

Expression

Bacterial

Promoter

Pbad and Plac

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pOPINE

Plasmid

#26043

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

His-lacZ-KHHHHHH

Tags

KHHHHHH and MAHHHHHHSSG

Expression

Bacterial, Insect, and Mamm…

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pBAD-D2

Plasmid

#37470

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D2 second generation cameleon (calcium sensor)

Expression

Bacterial

Promoter

Ara

Available Since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
p236

Plasmid

#186182

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 236

Use

Synthetic Biology; Cloning

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:GFP-CAAX

Plasmid

#184813

Purpose

Used to generate the Tg(QUAS:GFP-CAAX)c591 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GFP-CAAX-pA

Use

Zebrafish tol2 transgenesis

Tags

GFP-CAAX

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-tdTomato-WPRE

Plasmid

#104112

Purpose

AAV-mediated expression of tdTomato under the TRE promoter.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

tdTomato

Use

AAV

Promoter

TRE

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pK-myc-Par6B

Plasmid

#15473

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Insert

Partitioning-defective protein 6B (Pard6b Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
pENTR-SYNZIP17

Plasmid

#80671

Purpose

Encodes the synthetic coiled coil peptide SYNZIP17

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP17

Use

Entry vector

Available Since

Nov. 23, 2018

Availability

Academic Institutions and Nonprofits only

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p126

Plasmid

#186176

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 126

Use

Synthetic Biology; Cloning

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCL20C-ddIPpoI

Plasmid

#49053

Purpose

Expresses the restriction enzyme IPpoI under the control of Shield1

Depositor

Michael Kastan

Article

Goldstein et al Proc Natl Acad Sci U S A. 2013 Sep 30.

Insert

DD-HA-ER-IPpoI

Use

Lentiviral

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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JB85

Plasmid

#128909

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab9ASN-BirA*-HA-MAO (RAB9A Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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GW1-Peredox-mCherry-NLS

Plasmid

#32381

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry-NLS

Tags

Nuclear Localization Seq

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pKmK.I3R

Plasmid

#125065

Purpose

Storage plasmid for K.marxianus right homology arm

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Right homology arm for targeting integration to SWF1/ARO1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.I1R

Plasmid

#125063

Purpose

Storage plasmid for K.marxianus right homology arm

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Right homology arm for targeting integration to LAC4

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.I1L

Plasmid

#125030

Purpose

Storage plasmid for K.marxianus left homology arm; type 1 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Left homology arm for targeting integration to LAC4

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.I3L

Plasmid

#125032

Purpose

Storage plasmid for K.marxianus left homology arm; type 1 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Left homology arm for targeting integration to SWF1/ARO1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJBM4

Plasmid

#225291

Purpose

E. coli-Campylobacter shuttle vector; TetR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Tet(O) and its putative promoter

Expression

Bacterial

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pYM70

Plasmid

#119922

Purpose

CaHygB plasmids that facilitate genetic manipulations in the fungal pathogen

Depositor

Brian Wong

Article

Basso LR et al Yeast. 2010 Dec;27(12):1039-48. doi: 10.

Insert

CaHygB

Tags

CaHygB

Promoter

TEF2

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pALS3-Ma-sfGFP-WT

Plasmid

#212119

Purpose

Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

Depositor

Ryan Mehl

Inserts

SUMO-sfGFP-WT
M. alvus Pyl-tRNA(6)

Tags

His6

Expression

Bacterial

Promoter

araC and lpp

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pETM43 p62 FL (1-440)

Plasmid

#210425

Purpose

Bacterial expression of MBP-p62 FL (1-440) with 3C cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 FL (NUP62 Human)

Tags

6xHis and MBP

Expression

Bacterial

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pKmK.I2R

Plasmid

#125064

Purpose

Storage plasmid for K.marxianus right homology arm

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Right homology arm for targeting integration to chromosome V: 23743..21744

Use

Synthetic Biology

Expression

Yeast

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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mCitrine-C1

Plasmid

#54587

Purpose

Localization: C1 Cloning Vector, Excitation: 516, Emission: 529

Depositor

Robert Campbell, Michael Davidson, Oliver Griesbeck, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Tags

mCitrine

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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PP7 guide

Plasmid

#86168

Purpose

Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

PP7 guide

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pKmK.I4R

Plasmid

#125066

Purpose

Storage plasmid for K.marxianus right homology arm

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Right homology arm for targeting integration to chromosome IV:240042..241741

Use

Synthetic Biology

Expression

Yeast

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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p166

Plasmid

#186180

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 166

Use

Synthetic Biology; Cloning

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p256

Plasmid

#186184

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 256

Use

Synthetic Biology; Cloning

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-CPDSalI

Plasmid

#38251

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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