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pDN626

Plasmid

#175380

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, URA3-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRMCD122

Plasmid

#172963

Purpose

pRMCD77 encoding Wzz mutant I35C/M32T

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz I35C/M32T mutant gene

Expression

Bacterial

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD112

Plasmid

#172958

Purpose

pRMCD77 encoding Wzz mutant G305A/G309A

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz G305A/G309A mutant gene

Expression

Bacterial

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pG-04

Plasmid

#166935

Purpose

Target DC (G)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

pT181 dual control activator (DC) - Flanked by BsaI restriction sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pG-03

Plasmid

#166934

Purpose

Target AD1.A6 part (G)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Star sense AD1.S6 - Block G- Flanked by BsaI sites

Use

Synthetic Biology

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pC-04

Plasmid

#166928

Purpose

STAR-DC part (C)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Dual control activator -Block C- Flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRF005d

Plasmid

#156327

Purpose

Expresses heterologous rRNA

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

rRNA operon, orthogonal-antiRBS

Use

Synthetic Biology

Mutation

WT antiRBS engineered to orthogonal antiRBS, PS e…

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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AlACR2_EYFP_pcDNA3.1

Plasmid

#161023

Purpose

Expresses AlACR2 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

AlACR2

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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AlACR3_EYFP_pcDNA3.1

Plasmid

#161024

Purpose

Expresses AlACR3 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

AlACR3

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSKAP

Plasmid

#122702

Purpose

Designed for the incorporation of DNA barcode sequences for subsequent allelic exchange on bacterial chromosomes.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Type

Empty backbone

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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spoTD297A_NS1

Plasmid

#100915

Purpose

expresses spoTD297A (from native promoter) from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

synpcc7942_1377 (spoT)

Use

Cyanobacteria cloning vector

Mutation

Changed D 297 to A

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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relA+_NS1

Plasmid

#100916

Purpose

expresses E.coli relA+ from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

RelA fragment from E.coli

Use

Cyanobacteria cloning vector

Tags

FLAG

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMX234

Plasmid

#23006

Depositor

Linda Wordeman

Article

Stumpff et al Dev Cell. 2008 Feb . 14(2):252-62.

Insert

mRFP-CenpB

Tags

mRFP1.0

Expression

Mammalian

Mutation

deleted CenpB amino acids 169-606, T145S

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(del6) AcAc

Plasmid

#111332

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

3' splice site AcAc

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(WTdel6)

Plasmid

#111324

Purpose

for T7 transcription of wt actin pre-mRNA (del6)

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(AcAc)

Plasmid

#111327

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

3' splice site AcAc mutation in intron

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(BrG)

Plasmid

#111326

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

branch site A to G mutation in intron

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(BrC)

Plasmid

#111325

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

branch site A to C mutation in intron

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pRS303 prp8-1

Plasmid

#111306

Purpose

prp8-1

Depositor

Christine Guthrie

Article

Umen et al Genetics. 1996 Jun;143(2):723-39.

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

prp8-1 ( G1801D )

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pJU225 Prp8-TAP

Plasmid

#111305

Purpose

Prp8-TAP

Depositor

Christine Guthrie

Article

Umen et al Genetics. 1996 Jun;143(2):723-39.

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-PH(Btk)KD(Akt1)

Plasmid

#86636

Purpose

Expresses mCherry-tagged chimeric fusion of Btk PH domain with the kinase domain of human Akt1

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

PH(Btk)KD(Akt1)

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pNR163

Plasmid

#79544

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pNR165

Plasmid

#79646

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNR187

Plasmid

#79553

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221 Tma16

Plasmid

#63779

Purpose

yeast ORF Tma16 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Tma16 (TMA16 Budding Yeast)

Use

Gateway donor vector

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNR164

Plasmid

#79545

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL00002

Plasmid

#50347

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Linker for joining modules with 3' TTCG overhangs to modules with 5' GTGG overhangs

Use

Plant expression

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL00001

Plasmid

#50346

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Linker for joining modules with 3' GTGG overhangs to modules with 5' TTCG overhangs

Use

Plant expression

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGI000

Plasmid

#48863

Purpose

Empty entry vector for creating GreenGate N-terminal tag + coding sequence + C-terminal tag modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGE003

Plasmid

#48854

Purpose

Provides the At1g04880 terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

At1g04880 terminator

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGD006

Plasmid

#48836

Purpose

Provides the SV40 nuclear localization signal as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

SV40 NLS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGE002

Plasmid

#48840

Purpose

Provides the WUS terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

WUS terminator

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGB006

Plasmid

#48823

Purpose

Provides an ER signal sequence as GreenGate N-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

signal sequence ER

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGC014

Plasmid

#48827

Purpose

Provides GFP as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

GFP

Use

Golden gate compatible cloning vector

Mutation

A206K

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL-2AA3

Plasmid

#41480

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

2AA3

Use

Pcr cloning vector

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
TAL-2AC3

Plasmid

#41481

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

2AC3

Use

Pcr cloning vector

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
TAL-2AG3

Plasmid

#41482

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

2AG3

Use

Pcr cloning vector

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
TAL-2AT3

Plasmid

#41483

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

2AT3

Use

Pcr cloning vector

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
TAL-2CA3

Plasmid

#41484

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

2CA3

Use

Pcr cloning vector

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
TAL-2CC3

Plasmid

#41485

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

2CC3

Use

Pcr cloning vector

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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