We narrowed to 8,624 results for: FIE

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pLY100-RetroEFS-HER2-28BBz-WPRE

Plasmid

#192201

Purpose

Retro-HER2CAR

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-HER2CAR (ERBB2 Synthetic)

Use

Retroviral; Mammalian expression

Mutation

NA

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

Plasmid

#67981

Purpose

Cas9 activity reporter (control) with mCherry and GFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_JAK2_WT

Plasmid

#81756

Purpose

Gateway Donor vector containing JAK2 , part of the Target Accelerator Plasmid Collection. *Please note this is not a true WT JAK2 sequence and contains a V617F mutation.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

JAK2 (JAK2 Human)

Use

Gateway entry vector

Mutation

V617F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-SMARCA4

Plasmid

#65391

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SMARCA4 (SMARCA4 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA5_miniTurbo-C12orf49

Plasmid

#155111

Purpose

transfection plasmid for the exogneous expression of N-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

C12orf49 (SPRING1 Human)

Tags

miniTurbo

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_DLL1_WT

Plasmid

#82108

Purpose

Gateway Donor vector containing DLL1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DLL1 (DLL1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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sgLATS1/2-2

Plasmid

#229432

Purpose

knockout of LATS1 and LATS2

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgLATS1/2-2 (LATS1, LATS2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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sgLATS1/2-1

Plasmid

#229431

Purpose

knockout of LATS1 and LATS2

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgLATS1/2-1 (LATS1, LATS2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry

Plasmid

#128327

Purpose

Reporter to evaluate YAP1/TEAD-mediated gene transcription

Depositor

Yutaka Hata

Article

Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

Inserts

H2B mCherry
YAP1 (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-Cas9Bsd-W

Plasmid

#68343

Purpose

Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-Cas9 cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Cas9 fused with Flag-tag and a NLS at the N termi…

Promoter

EF1a

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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TRIPZ-bioGEF-UBnc-PURO

Plasmid

#208044

Purpose

Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

UBnc (UBC Human)

Use

Lentiviral

Tags

Low-affinity Avitag

Mutation

L73P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_ATF4_WT

Plasmid

#82190

Purpose

Gateway Donor vector containing ATF4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ATF4 (ATF4 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN5(TA)-ires-puro

Plasmid

#167823

Purpose

control sensor for EKAREN5

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5(TA)

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRetroX-TetOne-Puro-p62

Plasmid

#204548

Purpose

Expresses p62 in a doxycycline-dependent manner in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

p62 (SQSTM1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_SETD8_WT

Plasmid

#82290

Purpose

Gateway Donor vector containing SETD8, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SETD8 (KMT5A Human)

Use

Gateway entry vector

Mutation

P316R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HAPLN1_WT

Plasmid

#81757

Purpose

Gateway Donor vector containing HAPLN1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HAPLN1 (HAPLN1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BMP2_WT

Plasmid

#82101

Purpose

Gateway Donor vector containing BMP2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BMP2 (BMP2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HIST1H1E_WT

Plasmid

#81762

Purpose

Gateway Donor vector containing HIST1H1E , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HIST1H1E (H1-4 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CDK4_p.R24C

Plasmid

#82175

Purpose

Gateway Donor vector containing CDK4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDK4 (CDK4 Human)

Use

Gateway entry vector

Mutation

R24C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NOTCH1_ICN

Plasmid

#82087

Purpose

Gateway Donor vector containing NOTCH1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NOTCH1 (NOTCH1 Human)

Use

Gateway entry vector

Mutation

ICN (AA1761-2556 present)

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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