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pCR3.1-cGK1

Plasmid

#106452

Purpose

expression of murine cyclic GMP-dependent kinase

Depositor

Georg Weber

Article

He et al Biochem Biophys Res Commun. 2004 Aug 13;

Insert

cyclic GMP-dependent kinase (Prkg1 Mouse)

Expression

Mammalian

Mutation

WT

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-RARG

Plasmid

#222570

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of RARG

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

RARG (RARG Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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DH-BB[5'SIN;181/25ORF]

Plasmid

#242479

Purpose

Helper plasmid to produce CHIKV-pseudotyped propogation incompetent Sindbis virus

Depositor

Justus Kebschull

Article

Kim et al bioRxiv 2025.06.25.661405; doi: https://

Insert

Chikungunya virus 181/25 strain structural proteins

Use

Sindbis virus helper construct

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLRD11

Plasmid

#208263

Purpose

Production of sclareol in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
abCAS
ssSS

Expression

Bacterial

Mutation

abCAS(D537A)

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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PB-U6insert

Plasmid

#104536

Purpose

A piggybac vector with a U6 promoter that allows cloning of guide RNAs

Depositor

George Church

Article

Kalhor et al Science. 2018 Aug 9. pii: science.aat980

Type

Empty backbone

Use

CRISPR, Mouse Targeting, and Synthetic Biology ; …

Expression

Mammalian

Promoter

U6

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-STRA8

Plasmid

#222585

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of STRA8, with hygromycin resistance

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

STRA8 (STRA8 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCR3.1-mutLuc

Plasmid

#106458

Purpose

luciferase reporter, CMV promoter (3 NFkB sites mutated)

Depositor

Georg Weber

Article

He et al Biochem Biophys Res Commun. 2004 Aug 13;

Insert

luciferase reporter, CMV promoter (3 NFkB sites mutated)

Use

Luciferase

Expression

Mammalian

Mutation

3 NFkB sites mutated

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

Plasmid

#118158

Purpose

CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS

Depositor

Jorge Ferrer

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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p4xEGFP-N1

Plasmid

#122164

Purpose

Expression of 4xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

4xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLRD14

Plasmid

#208266

Purpose

Production of syn-pimara-7,15-diene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL4

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD17

Plasmid

#208269

Purpose

Production of syn-stemod-13(17)-ene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL11

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_Z17-ehaA_LacI

Plasmid

#186313

Purpose

Plasmid for inducible E coli expression of the Z17 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

Z17-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pJRT.Agro.CLCrVA.009

Plasmid

#37972

Depositor

Niki Robertson

Article

Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi:

Insert

pJRTCLCrVA.009

Use

Agrobacterium binary

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pLRD04

Plasmid

#208258

Purpose

Production of ent-kaurene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
blr2150

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_Z18-ehaA_LacI

Plasmid

#186314

Purpose

Plasmid for inducible E coli expression of the Z18 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

Z18-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-SYMPK

Plasmid

#222556

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SYMPK

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SYMPK (SYMPK Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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SK-YFP-SPNM-B

Plasmid

#48661

Purpose

Bacterial SP and NM repression YFP reporter: protospacer B

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

SP/NM prototspacer B/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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p3xEGFP-N1

Plasmid

#122163

Purpose

Expression of 3xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

3xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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DS-ST1casN-

Plasmid

#48659

Purpose

Bacterial nuclease-null ST1 Cas9 expression

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9, nuclease-null
ST1 tracrRNA

Use

CRISPR

Mutation

D to A, DH to AA, N to A

Promoter

proC

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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p6xEGFP-N1

Plasmid

#122166

Purpose

Expression of 6xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

6xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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