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pJ211_rha_WT_low

Plasmid

#128851

Purpose

Low copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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dCas9-AsLOV2x3-NLSx3

Plasmid

#231566

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

dCas9-AsLOV2x3

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD18HMGR

Plasmid

#19041

Depositor

Jay Keasling

Article

Pitera et al Metab Eng. 2007 Mar . 9(2):193-207.

Insert

truncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase (HMG1 Budding Yeast)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pEN_TTmiRc2

Plasmid

#25752

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Gateway Cloning

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pWZL Hygro-NMYC TRF2 deltaB deltaM

Plasmid

#16355

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pNeuroD1-ERT2CreERT2

Plasmid

#237402

Purpose

Tamoxifen-dependent expression of ERT2CreERT2 in NeuroD1-positive cells

Depositor

Yusuke Kishi

Article

Sakai et al Development. 2025 Jul 1;152(13):dev20456

Insert

ERT2CreERT2

Expression

Mammalian

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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eNme2C-CBE6c

Plasmid

#215831

Purpose

Express CBE6c (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6c-eNme2C-2xUGI

Expression

Mammalian

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD011

Plasmid

#116852

Purpose

Expresses araC constitutively and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD011

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
mScarletI

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+_NLS-Tir1-HA

Plasmid

#117716

Purpose

mRNA synthesis of NLS-TIR1-HA for injection

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

TRANSPORT INHIBITOR RESPONSE 1

Tags

HA and NLS

Expression

Mammalian

Mutation

codon-optimized TIR1 for murine expression

Promoter

SP6

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221 Spinophilin

Plasmid

#87123

Purpose

Gateway donor vector for Spinophilin

Depositor

Anthony Baucum

Article

Hiday et al Neural Plast. 2017;2017:4153076. doi: 10

Insert

Spinophilin (PPP1R9B Human)

Use

Donor vector

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-AP-Ub-dGG

Plasmid

#232581

Purpose

Express Acceptor Peptide (AP) tagged Ubiquitin lacking the two terminal glycines

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Tags

AviTag

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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T444T-GFP(NCO)

Plasmid

#183972

Purpose

Enhanced RNAi clone for C. elegans knockdown of non-codon-optimized GFP

Depositor

David Matus

Article

Palmisano et al CourseSource, 2022

Insert

GFP (non-codon-optimized)

Use

RNAi

Expression

Worm

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-dsRed-recipient

Plasmid

#11658

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling dsRed expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

dsRed

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pGWB2-CYCD3;1

Plasmid

#140750

Purpose

Inducing plant cells to enter mitosis

Depositor

Bo Liu

Article

Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

Insert

Cyclin D3;1 (CYCD3;1 Mustard Weed)

Expression

Plant

Promoter

35S

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

Plasmid

#66708

Purpose

expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

Depositor

Charles Gersbach

Insert

S.Pyogenes VP64-dCas9-VP64

Use

Lentiviral and Synthetic Biology

Tags

VP64

Mutation

D10A and H840A

Promoter

hUbC

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-Uch37C88A

Plasmid

#19416

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

Uch37 (UCHL5 Human)

Tags

2XHA

Expression

Mammalian

Mutation

C88A

Available Since

Jan. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pARS3_MUbCAS9_GW

Plasmid

#128646

Purpose

T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a Gateway cassette for cloning gRNA

Depositor

G. Eric Schaller

Article

Worthen et al Development. 2019 Jun 3. pii: dev.174870

Type

Empty backbone

Tags

HA

Expression

Plant

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDIV297

Plasmid

#204820

Purpose

AMA1 plasmid with amdS selection marker

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Insert

amdS

Use

Fungal expression

Expression

Bacterial

Mutation

two silent mutations (nt177T>C, nt879C>T)

Promoter

Aspergillus nidulans trpC promoter

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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DT037A

Plasmid

#159398

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23101 (strong) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23101-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

J23101 (strong) promoter

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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LV-Floxed Luc

Plasmid

#60622

Purpose

Expresses a conditional luciferase cassette dependent on Cre mediated recombination

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

Floxed Luciferase

Use

Cre/Lox, Lentiviral, and Luciferase

Promoter

human UbC

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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